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<p>Strain H1-120<sup>T</sup> was isolated from a heap of mined rock at the Rozalia gold mine (Slovakia) during a study of heavy-metal-resistant microbiota. The cells are Gram-negative, oxidase- and catalase-positive, with optimal growth at 35&#xa0;°C, pH 6.5–7.5, and 0.5% (<i>w/v</i>) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences, together with phylogenomic analysis, placed strain H1-120<sup>T</sup> within the <i>Pollutimonas</i> genus, with <i>Pollutimonas subterranea</i> (98.2%), <i>Pollutimonas bauzanensis</i> (98.0%), and <i>Pollutimonas thiosulfatoxidans</i> (98.0%) as the closest related type strains. Digital DNA–DNA hybridisation, average nucleotide identity, and average amino acid identity values between strain H1-120<sup>T</sup> and its closely related type strains of the genera <i>Pollutimonas, Pusillimonas, Parapusillimonas, Allopusillimonas, Mesopusillimonas, Neopusillimonas, Eoetvoesiella</i> and <i>Candidimonas</i> ranged from 19.4% to 22.9%, 71.53% to 80.6%, and 65.47% to 78.72%, respectively. The predominant respiratory quinones were Q8 (98.0%), Q9 (1.2%), and Q7 (0.8%). The major fatty acids were C<sub>16:0</sub>, C<sub>18:1</sub> <i>ω</i>7c, and C<sub>17:0</sub> cyclo, together with summed feature 2 (iso-C<sub>16:1</sub> I/C<sub>14:0</sub> 3-OH and/or C<sub>12:0</sub> ALDE) and summed feature 3 (C<sub>16:1</sub> <i>ω</i>7c and/or C<sub>16:1</sub> <i>ω</i>6c). The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylethanolamine, together with unidentified aminophospholipids. The genome of strain H1-120<sup>T</sup> has a G + C content of 59.9&#xa0;mol% and a size of 4.6&#xa0;Mb. Based on the 16S rRNA gene phylogeny, genomic data, and phenotypic characteristics of strain H1-120<sup>T</sup>, a novel species with the name <i>Pollutimonas rozaliensis</i> sp. nov., is proposed. The type strain is H1-120<sup>T</sup> (= DSM 118324<sup>T</sup> = CCM 9437<sup>T</sup>).</p>

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Pollutimonas rozaliensis sp. nov., a novel bacterium isolated from gold ore of the active Rozalia mine (Central Europe, Slovakia)

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  • 发表日期：

    2026年3月26日

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  • Lea Nosalova,
  • Sona Brestovicova,
  • Jana Kiskova,
  • Tamas Felfoldi,
  • Anna Alexovic Matiasova,
  • Lenka Malinicova,
  • Mariana Kolesarova,
  • Peter Pristas

摘要

Strain H1-120T was isolated from a heap of mined rock at the Rozalia gold mine (Slovakia) during a study of heavy-metal-resistant microbiota. The cells are Gram-negative, oxidase- and catalase-positive, with optimal growth at 35 °C, pH 6.5–7.5, and 0.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences, together with phylogenomic analysis, placed strain H1-120T within the Pollutimonas genus, with Pollutimonas subterranea (98.2%), Pollutimonas bauzanensis (98.0%), and Pollutimonas thiosulfatoxidans (98.0%) as the closest related type strains. Digital DNA–DNA hybridisation, average nucleotide identity, and average amino acid identity values between strain H1-120T and its closely related type strains of the genera Pollutimonas, Pusillimonas, Parapusillimonas, Allopusillimonas, Mesopusillimonas, Neopusillimonas, Eoetvoesiella and Candidimonas ranged from 19.4% to 22.9%, 71.53% to 80.6%, and 65.47% to 78.72%, respectively. The predominant respiratory quinones were Q8 (98.0%), Q9 (1.2%), and Q7 (0.8%). The major fatty acids were C16:0, C18:1 ω7c, and C17:0 cyclo, together with summed feature 2 (iso-C16:1 I/C14:0 3-OH and/or C12:0 ALDE) and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylethanolamine, together with unidentified aminophospholipids. The genome of strain H1-120T has a G + C content of 59.9 mol% and a size of 4.6 Mb. Based on the 16S rRNA gene phylogeny, genomic data, and phenotypic characteristics of strain H1-120T, a novel species with the name Pollutimonas rozaliensis sp. nov., is proposed. The type strain is H1-120T (= DSM 118324T = CCM 9437T).