Scholarlink

<p>This study focuses on the isolation, identification, and characterization of <i>Enterococcus faecalis</i> strain Ef-01 and the bacteriophage vB_EfS_L1 targeting it. <i>E. faecalis</i> was isolated from hospital laboratory samples and confirmed through PCR analysis, yielding a 1466&#xa0;bp sequence with high homology to <i>E. faecalis</i> ATCC 19433. Antibiotic sensitivity testing revealed that Ef-01 was susceptible to six antibiotics including ciprofloxacin and linezolid, intermediately susceptible to four, and resistant to five antibiotics such as penicillin and cefradine. Subsequently, bacteriophage vB_EfS_L1 was isolated from hospital wastewater and purified through multiple rounds of plaque purification. The highest titer, reaching 4.0 × 10<sup>11</sup> PFU/mL, was achieved when propagation was carried out at a multiplicity of infection (MOI) of 0.01. Host range analysis revealed that phage vB_EfS_L1 lysed 40% of clinical <i>Enterococcus</i> isolates, including multidrug-resistant strains, demonstrating a moderate yet clinically relevant tropism. Further analysis showed that vB_EfS_L1 exhibited excellent stability at temperatures from −20 to 37&#xa0;°C, but was inactivated at 80&#xa0;°C. It also maintained activity across a pH range of 4–10, with the highest activity at acidic pH values. The bacteriophage displayed resistance to chloroform and ether but was sensitive to SDS and proteinase K. Additionally, the bacteriophage was UV-sensitive, with a rapid decline in titer upon prolonged exposure. The genome of vB_EfS_L1, consisting of 41.151&#xa0;bp, was analyzed, revealing genes related to structural components, DNA replication, host lysis, and packaging. Functional gene predictions confirmed the absence of lysogeny or virulence-associated genes, ensuring the safety of vB_EfS_L1 for potential therapeutic applications. These findings highlight the bacteriophage’s potential as a therapeutic agent against <i>E. faecalis</i>, contributing to the development of phage-based interventions in bacterial infections.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biological characteristics and genome analysis of Enterococcus faecalis phage vB_EfS_L1

  • 期刊论文

  • 发表日期：

    2026年4月7日

查看全文
  • Huijun Geng,
  • Yong Chang,
  • Yanqiu Zhou,
  • Zhe Yu,
  • Xiaobo Wang,
  • Yongping Xu

摘要

This study focuses on the isolation, identification, and characterization of Enterococcus faecalis strain Ef-01 and the bacteriophage vB_EfS_L1 targeting it. E. faecalis was isolated from hospital laboratory samples and confirmed through PCR analysis, yielding a 1466 bp sequence with high homology to E. faecalis ATCC 19433. Antibiotic sensitivity testing revealed that Ef-01 was susceptible to six antibiotics including ciprofloxacin and linezolid, intermediately susceptible to four, and resistant to five antibiotics such as penicillin and cefradine. Subsequently, bacteriophage vB_EfS_L1 was isolated from hospital wastewater and purified through multiple rounds of plaque purification. The highest titer, reaching 4.0 × 1011 PFU/mL, was achieved when propagation was carried out at a multiplicity of infection (MOI) of 0.01. Host range analysis revealed that phage vB_EfS_L1 lysed 40% of clinical Enterococcus isolates, including multidrug-resistant strains, demonstrating a moderate yet clinically relevant tropism. Further analysis showed that vB_EfS_L1 exhibited excellent stability at temperatures from −20 to 37 °C, but was inactivated at 80 °C. It also maintained activity across a pH range of 4–10, with the highest activity at acidic pH values. The bacteriophage displayed resistance to chloroform and ether but was sensitive to SDS and proteinase K. Additionally, the bacteriophage was UV-sensitive, with a rapid decline in titer upon prolonged exposure. The genome of vB_EfS_L1, consisting of 41.151 bp, was analyzed, revealing genes related to structural components, DNA replication, host lysis, and packaging. Functional gene predictions confirmed the absence of lysogeny or virulence-associated genes, ensuring the safety of vB_EfS_L1 for potential therapeutic applications. These findings highlight the bacteriophage’s potential as a therapeutic agent against E. faecalis, contributing to the development of phage-based interventions in bacterial infections.