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<p>The fungus <i>Aspergillus flavus</i> is one of the most important mycotoxigenic species that produces potent carcinogenic secondary metabolites called aflatoxins, and its harmful ability has a considerable negative impact on human and livestock health. This study assessed an antifungal activity against <i>A. flavus</i> and evaluated <i>in vitro</i> probiotic properties of lactic acid bacteria newly isolated from the digestive tract of two popular edible crickets, <i>Gryllus bimaculatus</i> (GL) and <i>Acheta domesticus</i> (AL). Moreover, metabolic profiles including anti-<i>A. flavus</i> compounds produced by promising lactic acid bacteria were investigated. In total of 22 isolates, four lactic acid bacterial candidates comprising AL01, GL01, GL05, and GL11 exhibited inhibitory effect on mycelial growth and anti-fungal spore germination of <i>Aspergillus flavus</i> TISTR 3366. Based on molecular analysis of 16S rRNA gene sequences and biochemical tests using API 50 CHL, these isolates were identified as <i>Lacticaseibacillus paracasei</i> (AL01), <i>Lacticaseibacillus rhamnosus</i> (GL01, and GL11), and <i>Pediococcus pentosaceus</i> (GL05). Among them, two potential strains comprising <i>Lb. paracasei</i> AL01 and <i>P. pentosaceus</i> GL05 showed the potent probiotic characteristics based on bacterial viability in gastrointestinal tract conditions, good cell surface properties, and bacterial safety. Metabolic profiles of the cell-free supernatant (CFS) obtained from AL01- and GL05-cultures were investigated using gas chromatography mass spectrometry (GC-MS), and revealed a complex mixture of bioactive compounds, including short-chain hydrocarbons, organic acids, and other antifungal metabolites. Overall results suggested that the lactic acid bacteria obtained from edible crickets can be a promising probiotic and biocontrol agent against <i>A. flavus</i>. The finding in this study benefits for biologically reducing mycotoxicity of harmful fungi in food and agriculture.</p> Graphical abstract <p></p>

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Anti-Aspergillus flavus activity and probiotic properties of lactic acid bacteria isolated from the digestive tract of edible crickets

  • 期刊论文

  • 发表日期：

    2026年3月18日

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  • Kittipong Chanworawit,
  • Pongphara Sueto,
  • Pinsurang Deevong

摘要

The fungus Aspergillus flavus is one of the most important mycotoxigenic species that produces potent carcinogenic secondary metabolites called aflatoxins, and its harmful ability has a considerable negative impact on human and livestock health. This study assessed an antifungal activity against A. flavus and evaluated in vitro probiotic properties of lactic acid bacteria newly isolated from the digestive tract of two popular edible crickets, Gryllus bimaculatus (GL) and Acheta domesticus (AL). Moreover, metabolic profiles including anti-A. flavus compounds produced by promising lactic acid bacteria were investigated. In total of 22 isolates, four lactic acid bacterial candidates comprising AL01, GL01, GL05, and GL11 exhibited inhibitory effect on mycelial growth and anti-fungal spore germination of Aspergillus flavus TISTR 3366. Based on molecular analysis of 16S rRNA gene sequences and biochemical tests using API 50 CHL, these isolates were identified as Lacticaseibacillus paracasei (AL01), Lacticaseibacillus rhamnosus (GL01, and GL11), and Pediococcus pentosaceus (GL05). Among them, two potential strains comprising Lb. paracasei AL01 and P. pentosaceus GL05 showed the potent probiotic characteristics based on bacterial viability in gastrointestinal tract conditions, good cell surface properties, and bacterial safety. Metabolic profiles of the cell-free supernatant (CFS) obtained from AL01- and GL05-cultures were investigated using gas chromatography mass spectrometry (GC-MS), and revealed a complex mixture of bioactive compounds, including short-chain hydrocarbons, organic acids, and other antifungal metabolites. Overall results suggested that the lactic acid bacteria obtained from edible crickets can be a promising probiotic and biocontrol agent against A. flavus. The finding in this study benefits for biologically reducing mycotoxicity of harmful fungi in food and agriculture.

Graphical abstract