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<p>This study describes cyanobacterial diversity and toxicity of <i>Microcystis aeruginosa</i> detected in Umiam Lake and Ward’s Lake of Meghalaya, India. The WGS analyses of one-time enriched lake water sample in BG-11 medium showed the presence of many cyanobacteria including harmful <i>Microcystis aeruginosa</i>. The PCR using DNA extracted directly from lake water showed the presence of microcystin producing gene (<i>mcyA)</i>. Two <i>Microcystis</i> spp were isolated from Umiam Lake water (25° 39′ 11.52" N 91° 53′ 3.48" E) and identified by 16S rDNA sequence analysis. The HPLC analysis revealed microcystin-RR in the methanolic crude extract from one of the isolates, which was further confirmed by LC–MS/MS analysis. The toxicity investigation of crude extract using male and female mice showed necrosis, and LD<sub>50</sub> values at 304&#xa0;mg/kg bw and 366&#xa0;mg/kg bw, respectively. Hepatic enzyme markers ALP, SGPT, and SGOT showed a sharp increase in toxin treated mice. Further, histology and scanning electron microscopy depicted marked changes in overall liver architecture, revealing the hepatotoxic nature of the crude extract. The overall result underscores the need for evaluation of toxin producing cyanobacteria in waterbodies across the NE region.</p>

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Microcystis aeruginosa detected in freshwater lakes in Meghalaya, India show strong hepatotoxicity

  • 期刊论文

  • 发表日期：

    2026年3月3日

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  • Waniabha J. Lyngdoh,
  • Langpoklakpam James Singh,
  • Ankita Dey,
  • Divya Newar,
  • Jopthiaw Nongdhar,
  • Surya Bhan,
  • Arvind Kumar Singh

摘要

This study describes cyanobacterial diversity and toxicity of Microcystis aeruginosa detected in Umiam Lake and Ward’s Lake of Meghalaya, India. The WGS analyses of one-time enriched lake water sample in BG-11 medium showed the presence of many cyanobacteria including harmful Microcystis aeruginosa. The PCR using DNA extracted directly from lake water showed the presence of microcystin producing gene (mcyA). Two Microcystis spp were isolated from Umiam Lake water (25° 39′ 11.52" N 91° 53′ 3.48" E) and identified by 16S rDNA sequence analysis. The HPLC analysis revealed microcystin-RR in the methanolic crude extract from one of the isolates, which was further confirmed by LC–MS/MS analysis. The toxicity investigation of crude extract using male and female mice showed necrosis, and LD50 values at 304 mg/kg bw and 366 mg/kg bw, respectively. Hepatic enzyme markers ALP, SGPT, and SGOT showed a sharp increase in toxin treated mice. Further, histology and scanning electron microscopy depicted marked changes in overall liver architecture, revealing the hepatotoxic nature of the crude extract. The overall result underscores the need for evaluation of toxin producing cyanobacteria in waterbodies across the NE region.