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<p>Agro-industrial wastes contain lignocellulose, of which, the hemicellulosic fraction can be utilized for the production of value-added products such as xylitol, leading to the generation of a cost-effective and sustainable bioprocess. Biomass pretreatment is necessary for efficient enzymatic hydrolysis, with alkaline pretreatment enhancing the bioavailability of fermentable sugars such as xylose from the hemicellulosic component of lignocellulosic biomass. In the current study, a xylanolytic <i>Aspergillus terreus</i> strain was exposed to the chemical mutagen EMS in an attempt to enhance the xylanase enzyme production, reaching 12.83 UmL⁻¹. Subsequently, upon optimization of process and parameters, at a pH of 6.5, a reaction temperature of 60&#xa0;°C, with the presence of 20 mM Zn<sup>2+</sup> metal ions, the xylanase activity further increased to 16.11 UmL⁻¹. Upon saccharification of sugarcane bagasse, 0.21 gg⁻¹ xylose yield was obtained, and <i>Yarrowia lipolytica</i> strain was used for the biotransformation of xylose to xylitol using the bagasse hydrolysate as nutrient source. A titer of 10.27 gL⁻¹ was achieved utilizing solely sugarcane bagasse derived xylose as a nutrient source, thereby facilitating the application of the <i>Y. lipolytica</i> strain for the bioremediation of lignocellulosic biomass and the production of value-added products.</p> Graphical Abstract <p></p>

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EMS-induced mutagenesis of Aspergillus terreus for enhanced xylanase production and subsequent biotransformation of sugarcane bagasse-derived xylose towards xylitol

  • 期刊论文

  • 发表日期：

    2026年2月2日

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  • Satwika Das,
  • Ramandeep Kaur,
  • Naveen Kumar Bhukya,
  • Kanimozhi Jeyaram,
  • Ashish A Prabhu

摘要

Agro-industrial wastes contain lignocellulose, of which, the hemicellulosic fraction can be utilized for the production of value-added products such as xylitol, leading to the generation of a cost-effective and sustainable bioprocess. Biomass pretreatment is necessary for efficient enzymatic hydrolysis, with alkaline pretreatment enhancing the bioavailability of fermentable sugars such as xylose from the hemicellulosic component of lignocellulosic biomass. In the current study, a xylanolytic Aspergillus terreus strain was exposed to the chemical mutagen EMS in an attempt to enhance the xylanase enzyme production, reaching 12.83 UmL⁻¹. Subsequently, upon optimization of process and parameters, at a pH of 6.5, a reaction temperature of 60 °C, with the presence of 20 mM Zn2+ metal ions, the xylanase activity further increased to 16.11 UmL⁻¹. Upon saccharification of sugarcane bagasse, 0.21 gg⁻¹ xylose yield was obtained, and Yarrowia lipolytica strain was used for the biotransformation of xylose to xylitol using the bagasse hydrolysate as nutrient source. A titer of 10.27 gL⁻¹ was achieved utilizing solely sugarcane bagasse derived xylose as a nutrient source, thereby facilitating the application of the Y. lipolytica strain for the bioremediation of lignocellulosic biomass and the production of value-added products.

Graphical Abstract