Scholarlink

<p><i>Vibrio parahaemolyticus</i> is a foodborne pathogen that can cause severe gastroenteritis. After entering the human intestine through contaminated seafood (1.00% NaCl) <i>V. parahaemolyticus</i> will encounter a physiologically related dual pressure environment: low salinity and elevated bile salts (0.03%-0.30%). Although bile salts can affect <i>V. parahaemolyticus</i> under optimal salinity conditions (3.00% NaCl), little is known about their effects on paralysis under low salt conditions (0.90% NaCl) in the intestinal stress environment. This research uniquely simulated this intestinal niche using 0.90% NaCl-0.10% bile salts, revealing its effects on growth kinetics, motility, biofilm formation, and transcriptome responses. The main findings include: significant inhibition of growth (prolonged the lag time (LT)), decreased the maximum specific growth rate (<i>µ</i><sub>max)</sub>), swimming ability, and biofilm formation; But it enhances the ability to swarming; And unique transcriptome reprogramming. In addition, transcriptome sequencing revealed that swarming related genes, biofilm related genes, and T3SS virulence genes were significantly down regulated, while iron metabolism and swimming related genes were significantly up-regulated. It is crucial that KEGG enrichment indicates that the ribosomal pathway may be the central regulatory hub for observed biofilm and motility inhibition. This research provides the first comprehensive analysis of the effects of bile salts on intestinal related low salinity, providing important insights into the intestinal adaptation and pathogenic mechanisms of <i>V. parahaemolyticus</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The effect of bile salts stress on the biology and transcriptome characteristics of Vibrio parahaemolyticus under low salt environment

  • 期刊论文

  • 发表日期：

    2026年2月16日

查看全文
  • Zhiyong Song,
  • Ran Zhang,
  • Shiying Ji,
  • Zhengchuan Long,
  • Yingjie Pan,
  • Qingchao Xie,
  • Yong Zhao,
  • Haiquan Liu

摘要

Vibrio parahaemolyticus is a foodborne pathogen that can cause severe gastroenteritis. After entering the human intestine through contaminated seafood (1.00% NaCl) V. parahaemolyticus will encounter a physiologically related dual pressure environment: low salinity and elevated bile salts (0.03%-0.30%). Although bile salts can affect V. parahaemolyticus under optimal salinity conditions (3.00% NaCl), little is known about their effects on paralysis under low salt conditions (0.90% NaCl) in the intestinal stress environment. This research uniquely simulated this intestinal niche using 0.90% NaCl-0.10% bile salts, revealing its effects on growth kinetics, motility, biofilm formation, and transcriptome responses. The main findings include: significant inhibition of growth (prolonged the lag time (LT)), decreased the maximum specific growth rate (µmax)), swimming ability, and biofilm formation; But it enhances the ability to swarming; And unique transcriptome reprogramming. In addition, transcriptome sequencing revealed that swarming related genes, biofilm related genes, and T3SS virulence genes were significantly down regulated, while iron metabolism and swimming related genes were significantly up-regulated. It is crucial that KEGG enrichment indicates that the ribosomal pathway may be the central regulatory hub for observed biofilm and motility inhibition. This research provides the first comprehensive analysis of the effects of bile salts on intestinal related low salinity, providing important insights into the intestinal adaptation and pathogenic mechanisms of V. parahaemolyticus.