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<p>Two bacterial strains were isolated from root nodules of the chickpea plant (<i>Cicer arietinum</i>) in West Bengal and characterized to assess their potential for heavy metal (HM) tolerance and plant growth-promoting (PGP) attributes. Phylogenetic analysis based on the 16&#xa0;S rRNA gene identified these strains, SMAJ_63 and SMAJ_180, belonging to the genera <i>Enterobacter</i> sp. and <i>Labrys</i> sp, respectively. The two strains were screened for tolerance to multiple HMs, including arsenic (As), cadmium (Cd), cobalt (Co), chromium (Cr), and copper (Cu), with particular emphasis on chromium (Cr) due to its high environmental relevance and comparatively limited exploration in the literature. Two strains, SMAJ_63 and SMAJ_180, were evaluated for their tolerance to HMs, and their IC<sub>50</sub> values were determined. PGP attributes, including phosphate solubilization, indole acetic acid (IAA), and ammonia production were determined. Strain SMAJ_63 exhibited IAA production of 23.29&#xa0;µg/mL, whereas strain SMAJ_180 did not produce any detectable IAA. However, both strains were capable of producing ammonia, with strain SMAJ_63 and strain SMAJ_180 yielding 27.24 mM and 0.75 mM, respectively. Both strains demonstrated the ability to solubilize inorganic phosphate into soluble forms, indicating their potential contribution to enhanced phosphorus availability. Furthermore, strain SMAJ_63 exhibited exopolysaccharide (EPS) production, yielding 0.67&#xa0;g/L, whereas strain SMAJ_180 did not produce any detectable EPS. In conclusion, these findings highlight the potential of the selected strains as effective PGP bacteria under heavy metal stress, indicating their applicability in enhancing crop productivity and contributing to sustainable agricultural practices.</p>

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Isolation and characterization of root nodule-associated bacteria from chickpea (Cicer arietinum) for evaluating plant growth-promoting activities and heavy metal tolerance

  • 期刊论文

  • 发表日期：

    2025年12月1日

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  • Sudipta Majhi,
  • Mausumi Sikdar

摘要

Two bacterial strains were isolated from root nodules of the chickpea plant (Cicer arietinum) in West Bengal and characterized to assess their potential for heavy metal (HM) tolerance and plant growth-promoting (PGP) attributes. Phylogenetic analysis based on the 16 S rRNA gene identified these strains, SMAJ_63 and SMAJ_180, belonging to the genera Enterobacter sp. and Labrys sp, respectively. The two strains were screened for tolerance to multiple HMs, including arsenic (As), cadmium (Cd), cobalt (Co), chromium (Cr), and copper (Cu), with particular emphasis on chromium (Cr) due to its high environmental relevance and comparatively limited exploration in the literature. Two strains, SMAJ_63 and SMAJ_180, were evaluated for their tolerance to HMs, and their IC50 values were determined. PGP attributes, including phosphate solubilization, indole acetic acid (IAA), and ammonia production were determined. Strain SMAJ_63 exhibited IAA production of 23.29 µg/mL, whereas strain SMAJ_180 did not produce any detectable IAA. However, both strains were capable of producing ammonia, with strain SMAJ_63 and strain SMAJ_180 yielding 27.24 mM and 0.75 mM, respectively. Both strains demonstrated the ability to solubilize inorganic phosphate into soluble forms, indicating their potential contribution to enhanced phosphorus availability. Furthermore, strain SMAJ_63 exhibited exopolysaccharide (EPS) production, yielding 0.67 g/L, whereas strain SMAJ_180 did not produce any detectable EPS. In conclusion, these findings highlight the potential of the selected strains as effective PGP bacteria under heavy metal stress, indicating their applicability in enhancing crop productivity and contributing to sustainable agricultural practices.