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<p>The life cycle of <i>Ustilago maydis</i> involves three distinct morphological stages: sporidia, dikaryotic hyphae, and teliospores. While the yeast-like sporidia form is well described, the metabolic strategies enabling survival throughout its growth cycle remain unexplored. Here, we characterize the growth dynamics and the metabolic response of <i>U. maydis</i> in a YPD medium containing low (0.5%) or high (1.2%) glucose. Results indicate that the duration of exponential growth for <i>U. maydis</i> cells was the same in both media, with a specific growth rate of approximately 0.26&#xa0;h<sup>− 1</sup>. For <i>U. maydis</i> cells cultivated in YPD-0.5% medium, the transition to the stationary growth phase occurred between 72 and 96&#xa0;h, coinciding with a decrease in cellular biomass, a reduction in total RNA concentration, and diminished alternative oxidase (AOX) activity. Furthermore, <i>U. maydis</i> exhibited a short lifespan in the stationary phase, with most cells losing replicative capacity within 24–36&#xa0;h.</p>

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Changes in macromolecular content and AOX activity of Ustilago maydis: from exponential to stationary phase

  • 期刊论文

  • 发表日期：

    2025年10月18日

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  • Lucero Romero Aguilar,
  • Guadalupe Guerra Sánchez,
  • Oscar Flores Herrera,
  • Juan Luis Rendón,
  • Héctor Vázquez Meza,
  • Juan Pablo Pardo

摘要

The life cycle of Ustilago maydis involves three distinct morphological stages: sporidia, dikaryotic hyphae, and teliospores. While the yeast-like sporidia form is well described, the metabolic strategies enabling survival throughout its growth cycle remain unexplored. Here, we characterize the growth dynamics and the metabolic response of U. maydis in a YPD medium containing low (0.5%) or high (1.2%) glucose. Results indicate that the duration of exponential growth for U. maydis cells was the same in both media, with a specific growth rate of approximately 0.26 h− 1. For U. maydis cells cultivated in YPD-0.5% medium, the transition to the stationary growth phase occurred between 72 and 96 h, coinciding with a decrease in cellular biomass, a reduction in total RNA concentration, and diminished alternative oxidase (AOX) activity. Furthermore, U. maydis exhibited a short lifespan in the stationary phase, with most cells losing replicative capacity within 24–36 h.