Scholarlink

<p>This study examines the bacterial diversity and potential for secondary metabolite production of halophilic bacteria isolated from Oniru Beach, Lagos, Nigeria, a moderately saline marine environment. Using high-throughput next-generation sequencing, we profiled the bacterial community structure and complemented this with culture-dependent techniques to identify metabolite-producing strains. Physicochemical analysis revealed a slightly alkaline, oligotrophic, and low-oxygen environment enriched with bioactive metals, such as potassium, calcium, and iron, which may influence microbial adaptation and metabolic activity. Taxonomic profiling showed Proteobacteria (53.72%) as the dominant phylum, followed by Bacteroidetes (29.43%), Actinobacteria (3.88%), Deinococci (1.59%), and Firmicutes (1.37%), with Gammaproteobacteria (47.72%) being the most abundant class. Genus-level analysis highlighted <i>Acinetobacter</i>,<i> Chryseobacterium</i>,<i> Stenotrophomonas</i>,<i> Enterobacter</i>, and <i>Pseudomonas</i> as key constituents of this microbial community. Salt-tolerance assays and 16&#xa0;S rRNA sequencing identified highly halophilic isolates, including <i>Serratia marcescens</i>,<i> Staphylococcus edaphicus</i> and <i>Kurthia gibsonii</i>, which displayed diverse physiological and biochemical adaptations. Gas chromatography–mass spectrometry (GC-MS) revealed a wide range of secondary metabolites, including osmolytes, fatty acids, and sugar alcohols, underscoring the metabolic versatility of these isolates. Overall, this work demonstrates that Oniru Beach hosts a complex halophilic microbiome with specialized ecological adaptations and biotechnological potential, particularly for novel bioactive compound discovery and bioremediation strategies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Bacterial community structure and secondary metabolite insights from halophiles at Oniru Beach, Lagos

  • 期刊论文

  • 发表日期：

    2025年10月13日

查看全文
  • Abike Christianah Olaleye,
  • Habeebat Adekilekun Oyewusi,
  • Kolajo Adedamola Akinyede,
  • Oluwatosin Olubunmi Oladipo,
  • Bolaji Fatai Oyeyemi

摘要

This study examines the bacterial diversity and potential for secondary metabolite production of halophilic bacteria isolated from Oniru Beach, Lagos, Nigeria, a moderately saline marine environment. Using high-throughput next-generation sequencing, we profiled the bacterial community structure and complemented this with culture-dependent techniques to identify metabolite-producing strains. Physicochemical analysis revealed a slightly alkaline, oligotrophic, and low-oxygen environment enriched with bioactive metals, such as potassium, calcium, and iron, which may influence microbial adaptation and metabolic activity. Taxonomic profiling showed Proteobacteria (53.72%) as the dominant phylum, followed by Bacteroidetes (29.43%), Actinobacteria (3.88%), Deinococci (1.59%), and Firmicutes (1.37%), with Gammaproteobacteria (47.72%) being the most abundant class. Genus-level analysis highlighted Acinetobacter, Chryseobacterium, Stenotrophomonas, Enterobacter, and Pseudomonas as key constituents of this microbial community. Salt-tolerance assays and 16 S rRNA sequencing identified highly halophilic isolates, including Serratia marcescens, Staphylococcus edaphicus and Kurthia gibsonii, which displayed diverse physiological and biochemical adaptations. Gas chromatography–mass spectrometry (GC-MS) revealed a wide range of secondary metabolites, including osmolytes, fatty acids, and sugar alcohols, underscoring the metabolic versatility of these isolates. Overall, this work demonstrates that Oniru Beach hosts a complex halophilic microbiome with specialized ecological adaptations and biotechnological potential, particularly for novel bioactive compound discovery and bioremediation strategies.