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<p><i>Bacillus thuringiensis</i> (Bt) produces insecticidal crystal proteins and is widely used in pest control. Efficient strain selection for specific targets can be enhanced by integrating genomic and proteomic data. In this study, we sequenced 72 local Bt isolates and selected 12 for detailed proteomic and bioassay analyses. Expressed toxins were identified, and larval assays confirmed high toxicity in selected strains. Bt117 showed 16-fold higher toxicity against <i>Spodoptera frugiperda</i> compared to commercial strain <i>B. thuringiensis</i> serovar <i>kurstaki</i>, while Bt117 and Bt506 were similarly effective against <i>Helicoverpa armigera</i>. Comparative genomics revealed that vip3A expression is regulated by VipR, a finding confirmed experimentally. Phylogenetic analysis indicated that Bt117 and Bt202 are genomically divergent and more closely related to <i>Bacillus cereus</i>, suggesting horizontal gene transfer of pesticidal genes. Additionally, genes linked to plant growth-promoting traits (e.g., <i>asbA</i>, <i>ipdC</i>, and <i>accd</i>) were identified. This omics-guided strategy supports efficient Bt strain selection and broader application in sustainable agriculture.</p>

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Integrated genomic and proteomic analysis of local Bacillus thuringiensis isolates for targeted insect pest control and functional insight

  • 期刊论文

  • 发表日期：

    2025年7月11日

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  • Sumarin Soonsanga,
  • Amporn Rungrod,
  • Mongkon Utamatho,
  • Chutchanun Trakulnaleamsai,
  • Peeraphat Paenpong,
  • Wirulda Pootakham,
  • Narumon Phaonakrop,
  • Sittiruk Roytrakul,
  • Boonhiang Promdonkoy

摘要

Bacillus thuringiensis (Bt) produces insecticidal crystal proteins and is widely used in pest control. Efficient strain selection for specific targets can be enhanced by integrating genomic and proteomic data. In this study, we sequenced 72 local Bt isolates and selected 12 for detailed proteomic and bioassay analyses. Expressed toxins were identified, and larval assays confirmed high toxicity in selected strains. Bt117 showed 16-fold higher toxicity against Spodoptera frugiperda compared to commercial strain B. thuringiensis serovar kurstaki, while Bt117 and Bt506 were similarly effective against Helicoverpa armigera. Comparative genomics revealed that vip3A expression is regulated by VipR, a finding confirmed experimentally. Phylogenetic analysis indicated that Bt117 and Bt202 are genomically divergent and more closely related to Bacillus cereus, suggesting horizontal gene transfer of pesticidal genes. Additionally, genes linked to plant growth-promoting traits (e.g., asbA, ipdC, and accd) were identified. This omics-guided strategy supports efficient Bt strain selection and broader application in sustainable agriculture.