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<p>2-methylisoborneol (2-MIB) is one of the main cyanobacterial metabolites responsible for unpleasant taste and odor in drinking water. 2-MIB biosynthesis involves a two-step enzymatic reaction catalyzed by enzymes encoded by the <i>mtf</i> and <i>mtc</i> genes. Two homologous genes, <i>cnb</i>A and <i>cnb</i>B, which encode cyclic nucleotide-binding proteins of unknown function are located upstream and downstream of the <i>mtf</i> and <i>mtc</i> genes, respectively. This study investigated the involvement of <i>cnb</i> genes of 2-MIB-producing cyanobacterium <i>Pseudanabaena foetida</i> var. <i>intermedia</i> NIES 512 in 2-MIB synthesis using heterologous gene expression systems. Transgenic <i>Escherichia coli</i> and <i>Synechococcus elongatus</i> PCC 7942 strains expressing the <i>cnb</i>A<i>-mtf-mtc-cnb</i>B gene cluster exhibited a notable intracellular 2-MIB retention which was not shown by the transgenic strains expressing only the <i>mtf</i>-<i>mtc</i> gene unit. Immunoelectron microscopy detected that the Cnb proteins are associated with unknown intracellular compartments in transgenic <i>S</i>. <i>elongatus</i> PCC 7942 cells. This study suggests that 2-MIB-related Cnb proteins are involved in the formation of intracellular compartments, which are likely associated with intracellular 2-MIB retention.</p>

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Cnb proteins affect the intracellular 2-MIB retention in heterologous gene expression systems

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月11日

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  • Kaushalya Dayarathne,
  • Kimie Atsuzawa,
  • Toshiki Ishikawa,
  • Satoru Watanabe,
  • Yasuko Kaneko,
  • Aikeranmu Kadeer,
  • Masatoshi Yamaguchi,
  • Maki Kawai-Yamada

摘要

2-methylisoborneol (2-MIB) is one of the main cyanobacterial metabolites responsible for unpleasant taste and odor in drinking water. 2-MIB biosynthesis involves a two-step enzymatic reaction catalyzed by enzymes encoded by the mtf and mtc genes. Two homologous genes, cnbA and cnbB, which encode cyclic nucleotide-binding proteins of unknown function are located upstream and downstream of the mtf and mtc genes, respectively. This study investigated the involvement of cnb genes of 2-MIB-producing cyanobacterium Pseudanabaena foetida var. intermedia NIES 512 in 2-MIB synthesis using heterologous gene expression systems. Transgenic Escherichia coli and Synechococcus elongatus PCC 7942 strains expressing the cnbA-mtf-mtc-cnbB gene cluster exhibited a notable intracellular 2-MIB retention which was not shown by the transgenic strains expressing only the mtf-mtc gene unit. Immunoelectron microscopy detected that the Cnb proteins are associated with unknown intracellular compartments in transgenic S. elongatus PCC 7942 cells. This study suggests that 2-MIB-related Cnb proteins are involved in the formation of intracellular compartments, which are likely associated with intracellular 2-MIB retention.