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<p>In our previous study, a strain of <i>Pseudomonas composti</i> ODT-54 was isolated from the oyster digestive tissues. This strain is capable of producing Psl exopolysaccharides (EPS), which are directly involved in the specific binding to norovirus GII.6 P proteins. Here, we constructed an ODT-54 Psl EPS overexpression strain ODT-54 <i>psl</i><sup>(+)</sup> by substituting an exogenous arabinose-inducible promoter for the native promoter of the <i>psl</i> gene cluster. ODT-54 <i>psl</i><sup>(+)</sup> produced 50-70% more Psl EPS in FAB medium with 20&#xa0;g/L arabinose induction compared to ODT-54, according to ELISA analyses. Furthermore, a 50% increase in binding to GII.6 P proteins was observed for ODT-54 <i>psl</i><sup>(+)</sup>. These findings confirmed the success of the construction of the ODT-54 Psl EPS overexpression strain. This work provides important experimental material and helps further explore the structure of Psl EPS from <i>P. composti</i>.</p>

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Developing a mutant strain of Pseudomonas composti ODT-54 for enhanced production of Psl extracellular polysaccharide

  • 期刊论文

  • 发表日期：

    2025年3月7日

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  • Jieer Wu,
  • Feng Han,
  • Yongxin Yu,
  • Yongjie Wang

摘要

In our previous study, a strain of Pseudomonas composti ODT-54 was isolated from the oyster digestive tissues. This strain is capable of producing Psl exopolysaccharides (EPS), which are directly involved in the specific binding to norovirus GII.6 P proteins. Here, we constructed an ODT-54 Psl EPS overexpression strain ODT-54 psl(+) by substituting an exogenous arabinose-inducible promoter for the native promoter of the psl gene cluster. ODT-54 psl(+) produced 50-70% more Psl EPS in FAB medium with 20 g/L arabinose induction compared to ODT-54, according to ELISA analyses. Furthermore, a 50% increase in binding to GII.6 P proteins was observed for ODT-54 psl(+). These findings confirmed the success of the construction of the ODT-54 Psl EPS overexpression strain. This work provides important experimental material and helps further explore the structure of Psl EPS from P. composti.