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Background <p>The conventional identification of semen stains with the naked eye on the body of a&#xa0;victim of sexual assault can be difficult so that many samples are often taken at random from a&#xa0;victim’s body, with no guarantee of obtaining the perpetrator’s genetic material. With the STK® Sperm Tracker <i>STK Skin</i> (hereinafter <i>STK Skin</i>), AXO Science has introduced a&#xa0;non-toxic spray for the use on skin and hair, which can be used to visualize the acid phosphatase contained in human semen when illuminated with UV light (365 nm). The visualization with <i>STK Skin </i>does not alter the molecular analyses of collected semen stains.</p> Material and methods <p>For the validation of <i>STK Skin</i>, the specificity and sensitivity (among other things), shelf life/stability (in both full and partial half pack size of the dissolved solution) as well as possible influences on a&#xa0;subsequent molecular genetic analysis were examined. In addition, the performance was tested in simulated cases of sexual assault.</p> Results <p>The <i>STK Skin</i> showed a&#xa0;high sensitivity and specificity as well as a&#xa0;long shelf life. Visualization was still possible 24 h after application of semen samples; however, washing of affected areas did not result in the detection of any blue fluorescence. Vaginal secretions fluoresced after being sprayed with <i>STK Skin</i> and blood masks the fluorescence of acid phosphatase in a&#xa0;blood/semen mixture, while tested, e.g., care products did not interfere with the results. In realistically simulated test runs and all traces were successfully detected. Moreover, <i>STK Skin</i> showed no influence on the quality and/or quantity of the trace samples taken in further molecular analyses.</p> Conclusion <p>The <i>STK Skin</i> provides a&#xa0;high level of benefit in the detection of even small amounts of semen and the DNA profile of the trace donor could be determined even after months of sample storage.</p>

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STK® Sperm Tracker STK Skin verbessert die Detektion von Sperma auf der Haut und hilft bei der Untersuchung von Gewaltopfern

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月14日

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  • Maximilian Elischa Daniels,
  • Lisa Möhker,
  • Britta Gahr,
  • Petra Böhme

摘要

Background

The conventional identification of semen stains with the naked eye on the body of a victim of sexual assault can be difficult so that many samples are often taken at random from a victim’s body, with no guarantee of obtaining the perpetrator’s genetic material. With the STK® Sperm Tracker STK Skin (hereinafter STK Skin), AXO Science has introduced a non-toxic spray for the use on skin and hair, which can be used to visualize the acid phosphatase contained in human semen when illuminated with UV light (365 nm). The visualization with STK Skin does not alter the molecular analyses of collected semen stains.

Material and methods

For the validation of STK Skin, the specificity and sensitivity (among other things), shelf life/stability (in both full and partial half pack size of the dissolved solution) as well as possible influences on a subsequent molecular genetic analysis were examined. In addition, the performance was tested in simulated cases of sexual assault.

Results

The STK Skin showed a high sensitivity and specificity as well as a long shelf life. Visualization was still possible 24 h after application of semen samples; however, washing of affected areas did not result in the detection of any blue fluorescence. Vaginal secretions fluoresced after being sprayed with STK Skin and blood masks the fluorescence of acid phosphatase in a blood/semen mixture, while tested, e.g., care products did not interfere with the results. In realistically simulated test runs and all traces were successfully detected. Moreover, STK Skin showed no influence on the quality and/or quantity of the trace samples taken in further molecular analyses.

Conclusion

The STK Skin provides a high level of benefit in the detection of even small amounts of semen and the DNA profile of the trace donor could be determined even after months of sample storage.