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Introduction and Hypothesis <p>To compare the bladder microbiomes of participants with and without a gynecological cancer at the time of diagnostic surgery to determine their suitability as control participants in urobiome research studies.</p> Methods <p>IRB-approved clinical sample of continent adult female patients undergoing surgery for suspected gynecologic malignancy. Participants contributed clinical data (abstracted from the electronic medical record), and a catheterized urine sample obtained prior to antibiotic administration on the day of surgery. The study urine sample underwent DNA sequencing following amplification of hyper-variable region 4 (V4) of the bacterial 16S rRNA gene. The sequences were processed, annotated, and decontaminated. Alpha (within-sample) and beta (between-sample) diversity indices were computed, and the microbiome compositions across all participants were compared.</p> Results <p>Ninety-two women participated, including 20 with a gynecological cancer diagnosis and a comparison group of 72 without gynecologic cancer detected. The mean age was 53&#xa0;years old (range 22–92) with a mean BMI of 31&#xa0;kg/m<sup>2</sup> (26–38&#xa0;kg/m<sup>2</sup>); the majority (62%) were White. We observed no significant difference in the bladder microbiomes of the group with a cancer diagnosis and the controls.</p> Conclusions <p>Participants with a gynecological cancer diagnosis can be used as controls for bladder microbiome studies.</p>

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Gynecologic Oncology Patients have Urobiomes that are Similar to Women Without Gynecologic Cancer

  • 期刊论文

  • 发表日期：

    2025年7月29日

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  • Anisa Nasse,
  • Abigail Winder,
  • Melline Fontes Noronha,
  • Margaret R. Liotta,
  • Ronald K. Potkul,
  • Elizabeth R. Mueller,
  • Linda Brubaker,
  • Alan J. Wolfe

摘要

Introduction and Hypothesis

To compare the bladder microbiomes of participants with and without a gynecological cancer at the time of diagnostic surgery to determine their suitability as control participants in urobiome research studies.

Methods

IRB-approved clinical sample of continent adult female patients undergoing surgery for suspected gynecologic malignancy. Participants contributed clinical data (abstracted from the electronic medical record), and a catheterized urine sample obtained prior to antibiotic administration on the day of surgery. The study urine sample underwent DNA sequencing following amplification of hyper-variable region 4 (V4) of the bacterial 16S rRNA gene. The sequences were processed, annotated, and decontaminated. Alpha (within-sample) and beta (between-sample) diversity indices were computed, and the microbiome compositions across all participants were compared.

Results

Ninety-two women participated, including 20 with a gynecological cancer diagnosis and a comparison group of 72 without gynecologic cancer detected. The mean age was 53 years old (range 22–92) with a mean BMI of 31 kg/m2 (26–38 kg/m2); the majority (62%) were White. We observed no significant difference in the bladder microbiomes of the group with a cancer diagnosis and the controls.

Conclusions

Participants with a gynecological cancer diagnosis can be used as controls for bladder microbiome studies.