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<p>This study aims at determining the in-vitro biocompatibility of plasma-sprayed alumina (Al<sub>2</sub>O<sub>3</sub>)- and zirconia (ZrO<sub>2</sub>)-reinforced hydroxyapatite (HAP) coating on Ti6Al4V and SS316 substrates. It compares the biological implications of as-sprayed and heat-treated coatings, thus clarifying the means through which the performance of metallic implant coating can be improved.&#xa0;The NIH3T3 fibroblasts and human peripheral blood monocytes cells (hPBMCs) were seeded on the coatings for 48 and 96&#xa0;h. Cell viability was determined through both MTT and trypan blue tests and microscopic evaluations. The experiment compared the cell viability and reactive oxygen species (ROS) results between as-sprayed and heat-treated surfaces.&#xa0;Heat-treated coatings exhibited about 100% viability in both NIH3T3 and hPBMC cultures, compared to significant reduction in cell death (20–30% in NIH3T3, and 10–25% in hPBMCs) in as-sprayed coatings. ROS analysis showed that heat treatment was effective in reducing the oxidative stress, whereas as-sprayed surfaces stimulated the high levels of ROS. One-way ANOVA proved statistically significant positive changes in cell viability and ROS mitigation in all conditions of coating (p<InlineEquation ID="IEq1"> <EquationSource Format="TEX">\(\:&lt;\)</EquationSource> </InlineEquation> 0.001 in most cases). All results suggested that heat treatment enhanced the biocompatibility of reinforced HAP coating.&#xa0;This study presents a systematic comparison of biocompatibility of plasma-sprayed HAP coatings under different thermal conditions. The results highlighted the importance of heat treatment in optimizing the biological activity of metallic implant coatings, which is a promising direction of research in the future.</p>

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Enhancing biocompatibility: heat-treated Al2O3- and ZrO2- reinforced hydroxyapatite coatings on metallic implants

  • 期刊论文

  • 发表日期：

    2025年11月26日

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  • Amardeep Singh Kang,
  • Ali Basem,
  • Abhinav Kumar,
  • Manoj Kumar,
  • Dhouha Choukaier,
  • Patel Amishkumar Bhagvandas,
  • Ravinder Pal Singh,
  • Sergey Evgenievich Shcheklein,
  • Ankit Dilipkumar Oza

摘要

This study aims at determining the in-vitro biocompatibility of plasma-sprayed alumina (Al2O3)- and zirconia (ZrO2)-reinforced hydroxyapatite (HAP) coating on Ti6Al4V and SS316 substrates. It compares the biological implications of as-sprayed and heat-treated coatings, thus clarifying the means through which the performance of metallic implant coating can be improved. The NIH3T3 fibroblasts and human peripheral blood monocytes cells (hPBMCs) were seeded on the coatings for 48 and 96 h. Cell viability was determined through both MTT and trypan blue tests and microscopic evaluations. The experiment compared the cell viability and reactive oxygen species (ROS) results between as-sprayed and heat-treated surfaces. Heat-treated coatings exhibited about 100% viability in both NIH3T3 and hPBMC cultures, compared to significant reduction in cell death (20–30% in NIH3T3, and 10–25% in hPBMCs) in as-sprayed coatings. ROS analysis showed that heat treatment was effective in reducing the oxidative stress, whereas as-sprayed surfaces stimulated the high levels of ROS. One-way ANOVA proved statistically significant positive changes in cell viability and ROS mitigation in all conditions of coating (p \(\:<\) 0.001 in most cases). All results suggested that heat treatment enhanced the biocompatibility of reinforced HAP coating. This study presents a systematic comparison of biocompatibility of plasma-sprayed HAP coatings under different thermal conditions. The results highlighted the importance of heat treatment in optimizing the biological activity of metallic implant coatings, which is a promising direction of research in the future.