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<p>Benzo(a)pyrene (BaP) is the key archetypal of 16 priority polycyclic aromatic hydrocarbons (PAHs), exhibiting a severe hazard to both humans and ecosystems. Despite decades of research on microbial breakdown of PAHs, BaP biodegradation works are limited. <i>Bhargavaea beijingensis</i> BP14 isolated from earthworm <i>Perionyx excavates</i> gut, capable of BaP degradation by almost 50% within 14 days. To decipher the potential of the selected isolate, whole-genome sequence analysis was carried out. Presence of catechol 2,3-dioxygenase and ring cleavage dioxygenase gene in the genome of the <i>B. beijingensis</i> BP14 strain, showing its hydrocarbon degrading potentiality. The study indicated the utilization of a meta-cleavage pathway to biodegrade BaP by <i>B. beijingensis</i> BP14 strain through the catalysis of the enzyme catechol 2,3-dioxygenase, resulting in the production of phthalic acid as an intermediate. The primary metabolite detected through gas chromatography–mass spectrometry (GC–MS) investigation was identified as dibutyl phthalate. This study suggested that the biodegradation of BaP by <i>B. beijingensis</i> BP14 predominantly followed the phthalate pathway.</p>

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Biodegradation of Benzo[a]pyrene Using a Novel Gut Isolate Bhargavaea beijingensis BP14 from Perionyx excavatus

  • 期刊论文

  • 发表日期：

    2026年3月7日

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  • Nitu Gupta,
  • Raju Biswas,
  • Sandipan Banerjee,
  • Apurba Koley,
  • Rituparna Mukherjee,
  • Nandita Das,
  • Narayan Chandra Mandal,
  • Srinivasan Balachandran,
  • Raza Rafiqul Hoque

摘要

Benzo(a)pyrene (BaP) is the key archetypal of 16 priority polycyclic aromatic hydrocarbons (PAHs), exhibiting a severe hazard to both humans and ecosystems. Despite decades of research on microbial breakdown of PAHs, BaP biodegradation works are limited. Bhargavaea beijingensis BP14 isolated from earthworm Perionyx excavates gut, capable of BaP degradation by almost 50% within 14 days. To decipher the potential of the selected isolate, whole-genome sequence analysis was carried out. Presence of catechol 2,3-dioxygenase and ring cleavage dioxygenase gene in the genome of the B. beijingensis BP14 strain, showing its hydrocarbon degrading potentiality. The study indicated the utilization of a meta-cleavage pathway to biodegrade BaP by B. beijingensis BP14 strain through the catalysis of the enzyme catechol 2,3-dioxygenase, resulting in the production of phthalic acid as an intermediate. The primary metabolite detected through gas chromatography–mass spectrometry (GC–MS) investigation was identified as dibutyl phthalate. This study suggested that the biodegradation of BaP by B. beijingensis BP14 predominantly followed the phthalate pathway.