Scholarlink

<p>The Amazon hosts the world’s richest biodiversity but faces increasing anthropogenic pressures that threaten ecosystem health. To evaluate aquatic pollution impacts at molecular and biochemical levels, we analyzed DNA methylation, gene expression and oxidative stress biomarkers in gill, liver, and muscle tissues of the fish <i>Geophagus surinamensis</i> collected from Breves (reference), Abaetetuba, and Barcarena (industrial area). Individuals from Abaetetuba exhibited the strongest responses, with global hypermethylation in liver and muscle, decreased expression of <i>dnmt1</i>, <i>dnmt3b</i>, and <i>tet2</i>, and altered GST and LPO levels. Barcarena samples also showed increased methylation but milder transcriptional and biochemical changes, likely due to contaminant dispersion by Pará River hydrodynamics. The liver was the most responsive organ, reflecting its detoxification role. Overall, these findings demonstrate that pollution in Barcarena significantly affects nearby aquatic environments, highlighting the value of epigenetic and oxidative stress biomarkers as sensitive tools for environmental monitoring.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

DNA Methylation and Oxidative Stress in a Fish from an Area of the Amazon Historically Impacted by Anthropogenic Activities

  • 期刊论文

  • 发表日期：

    2025年11月2日

查看全文
  • Douglas dos Santos Marcelino,
  • Luana Beatriz Sales Pinon,
  • Yure Jefferson da Cruz do Nascimento,
  • Flávia dos Santos Tavares,
  • Luan Felipe da Silva Frade,
  • Maria Iracilda Cunha Sampaio,
  • Luis Adriano Santos do Nascimento,
  • Cesar Martins,
  • Lílian Lund Amado,
  • Adauto Lima Cardoso,
  • Renata Coelho Rodrigues Noronha

摘要

The Amazon hosts the world’s richest biodiversity but faces increasing anthropogenic pressures that threaten ecosystem health. To evaluate aquatic pollution impacts at molecular and biochemical levels, we analyzed DNA methylation, gene expression and oxidative stress biomarkers in gill, liver, and muscle tissues of the fish Geophagus surinamensis collected from Breves (reference), Abaetetuba, and Barcarena (industrial area). Individuals from Abaetetuba exhibited the strongest responses, with global hypermethylation in liver and muscle, decreased expression of dnmt1, dnmt3b, and tet2, and altered GST and LPO levels. Barcarena samples also showed increased methylation but milder transcriptional and biochemical changes, likely due to contaminant dispersion by Pará River hydrodynamics. The liver was the most responsive organ, reflecting its detoxification role. Overall, these findings demonstrate that pollution in Barcarena significantly affects nearby aquatic environments, highlighting the value of epigenetic and oxidative stress biomarkers as sensitive tools for environmental monitoring.