Aims/hypothesis <p>The chemokine receptor C-X-C chemokine receptor type&#xa0;3 (CXCR3) is a key chemoattractant molecule that facilitates the migration of activated T&#xa0;cells to the pancreas, leading to beta cell death. In this study, we investigated CXCR3 responses in B&#xa0;cells during type 1 diabetes progression.</p> Methods <p>Peripheral blood samples were obtained from individuals with recent-onset and long-duration type 1 diabetes, who were age- and sex-matched to non-diabetic donors. We isolated peripheral blood mononuclear cells (PBMCs) and examined changes in CXCR3 expression on lymphocytes from donors, performing multiparameter flow cytometry and functional cell culture assays. Human post-mortem pancreatic tissue was obtained from the Exeter Archival Diabetes Biobank. Immunofluorescence staining was used to assess CXCR3 expression in pancreatic tissues.</p> Results <p>We observed reduced CXCR3 expression on antigen-experienced B&#xa0;cells in individuals with a long duration of type 1 diabetes, although B&#xa0;cells remained responsive to IFNγ. In individuals who were recently diagnosed, IFNγ treatment resulted in increased CXCR3 expression compared with B&#xa0;cells from non-diabetic donors. B&#xa0;cells in pancreases that were recovered post-mortem from young recent-onset donors lacked CXCR3 expression, but co-staining to detect CD8⁺ T cells revealed a CXCR3⁺CD20⁺CD8⁺ T&#xa0;cell population, with their circulating counterpart showing increased CXCR3 expression.</p> Conclusions/interpretation <p>We conclude that the CXCR3 response in antigen-experienced B&#xa0;cells is dysregulated during the progression of type 1 diabetes. CXCR3 expression is limited in CD20⁺ B&#xa0;cells in pancreases from recent-onset individuals diagnosed with type 1 diabetes under 7 years of age, but evident on CD8⁺ T cells that express CD20.</p> Graphical Abstract <p></p>