Scholarlink

Key message <p>Based on GWAS, QTL mapping, BSA and RNA‒seq jointly reveal the genetic mechanisms underlying Verticillium wilt resistance in cotton and identify three candidate genes associated with this trait.</p> Abstract <p>Verticillium wilt, caused by the soil-borne vascular pathogen <i>Verticillium dahliae</i>, is a devastating disease that severely threatens global cotton production. However, the genetic basis of resistance to this disease remains poorly uncovered, hindering the advancement of genomics-assisted breeding programs for Verticillium wilt (VW) resistance. Here, we systematically evaluated VW resistance in a recombinant inbred line (RIL) population comprising 398 F<sub>8</sub> families and a backcross (BC) population comprising 325 <span>BC</span><sub>5</sub>F<sub>4</sub> families, under both greenhouse and field disease-nursery conditions. Based on the disease index, the RIL population were classified into four groups, with Group IV exhibiting the strongest resistance. Genome-wide association study (GWAS) identified 1299 significantly associated single-nucleotide polymorphism (SNP), of which 144 were repeatedly detected in two environments, and 17 were detected in three environments. Additionally, 20 stable quantitative trait loci (QTL) were identified across multiple environments. By integrating GWAS, QTL mapping and bulked segregant analysis (BSA), genetic loci on chromosomes A10 and A12 were jointly mapped. Furthermore, we pinpointed three key candidate resistance genes harboring nonsynonymous mutations. Among these, <i>GhHMT1</i><sup>Hap1</sup>, <i>GhβXT1</i><sup>Hap1</sup>, and <i>GhCYP7A15</i><sup>Hap2</sup> were defined as superior haplotypes; accessions carrying these haplotypes not only exhibited stronger resistance but also demonstrated a clear positive pyramiding effect. Virus-induced gene silencing (VIGS) of all three genes resulted in more severe Verticillium wilt symptoms in the silenced plants. In the <i>GhβXT1</i>-silenced plants, lignin content and callose deposition decreased, whereas H<sub>2</sub>O<sub>2</sub> content increased. Meanwhile, salicylic acid and jasmonic acid contents also decreased. Collectively, our study provides valuable genetic resources and candidate genes for breeding VW-resistant cotton cultivar and lays a theoretical foundation for elucidating the genetic regulatory mechanisms underlying this resistance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Multiple environmental test and genetic mapping jointly reveal genomic loci and associated genes underlying cotton Verticillium wilt resistance

  • 期刊论文

  • 发表日期：

    2026年7月18日

查看全文
  • Yalin Zhang,
  • Lihong Zhao,
  • Ziming Li,
  • Binhui Liu,
  • Junyuan Lv,
  • Zili Feng,
  • Feng Wei,
  • Jinglong Zhou,
  • Jiangping Han,
  • Yong Song,
  • Heqin Zhu,
  • Hongjie Feng,
  • Zhiying Ma

摘要

Key message

Based on GWAS, QTL mapping, BSA and RNA‒seq jointly reveal the genetic mechanisms underlying Verticillium wilt resistance in cotton and identify three candidate genes associated with this trait.

Abstract

Verticillium wilt, caused by the soil-borne vascular pathogen Verticillium dahliae, is a devastating disease that severely threatens global cotton production. However, the genetic basis of resistance to this disease remains poorly uncovered, hindering the advancement of genomics-assisted breeding programs for Verticillium wilt (VW) resistance. Here, we systematically evaluated VW resistance in a recombinant inbred line (RIL) population comprising 398 F8 families and a backcross (BC) population comprising 325 BC5F4 families, under both greenhouse and field disease-nursery conditions. Based on the disease index, the RIL population were classified into four groups, with Group IV exhibiting the strongest resistance. Genome-wide association study (GWAS) identified 1299 significantly associated single-nucleotide polymorphism (SNP), of which 144 were repeatedly detected in two environments, and 17 were detected in three environments. Additionally, 20 stable quantitative trait loci (QTL) were identified across multiple environments. By integrating GWAS, QTL mapping and bulked segregant analysis (BSA), genetic loci on chromosomes A10 and A12 were jointly mapped. Furthermore, we pinpointed three key candidate resistance genes harboring nonsynonymous mutations. Among these, GhHMT1Hap1, GhβXT1Hap1, and GhCYP7A15Hap2 were defined as superior haplotypes; accessions carrying these haplotypes not only exhibited stronger resistance but also demonstrated a clear positive pyramiding effect. Virus-induced gene silencing (VIGS) of all three genes resulted in more severe Verticillium wilt symptoms in the silenced plants. In the GhβXT1-silenced plants, lignin content and callose deposition decreased, whereas H2O2 content increased. Meanwhile, salicylic acid and jasmonic acid contents also decreased. Collectively, our study provides valuable genetic resources and candidate genes for breeding VW-resistant cotton cultivar and lays a theoretical foundation for elucidating the genetic regulatory mechanisms underlying this resistance.