Scholarlink

<p>Polyploidization is a pivotal process in plant evolution, serving as a major driving force for genome diversification and adaptive potential. However, the meiotic behavior of autoallopolyploid remains poorly understood. Here, we analyzed the meiotic behavior of a <i>Brassica</i> autoallohexaploid (A<sup>n</sup>A<sup>n</sup>C<sup>n</sup>C<sup>n</sup>C<sup>o</sup>C<sup>o</sup>) derived from <i>B. napus</i> (A<sup>n</sup>A<sup>n</sup>C<sup>n</sup>C<sup>n</sup>) and <i>B. oleracea</i> (C<sup>o</sup>C<sup>o</sup>), using fluorescence in situ hybridization (FISH). The addition of the C<sup>o</sup> genome from <i>B. oleracea</i> induced A-C homoeologous pairing in 78.5% of pollen mother cells (PMCs) in the S<sub>0</sub> generation. The prevalence of univalents, autotrivalents, and homoeologous pairings at diakinesis I strongly correlated with irregular chromosomal segregation at anaphase I. The frequency of homoeologous pairing was positively correlated with the extent of chromosomal collinearity. The emergence of a relatively stable euploid line in the S<sub>4</sub> generation suggests that successive selfing enhances meiotic stability in autoallohexaploids. Subsequently, we constructed a gene co-expression network based on differentially expressed genes (DEGs) between the stabilized S<sub>4</sub> line and the S<sub>0</sub> generation, which highlighted hub genes potentially governing meiotic stability. Together, these findings provide new insights into meiotic stability in autoallohexaploid and offer valuable knowledge for polyploid breeding and the improvement of genomic stability in <i>Brassica</i> crops.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Chromosomal collinearity drives meiotic instability in Brassica autoallopolyploid

  • 期刊论文

  • 发表日期：

    2026年7月9日

查看全文
  • Fang Yue,
  • Qinfei Li,
  • Jiaqin Mei,
  • Lei Wang,
  • Yixin Cui,
  • Kusum Rana,
  • Fajing Zheng,
  • Junxiong Xu,
  • Chunlei Shu,
  • Hong Zhang,
  • Zhiyong Xiong,
  • Wei Qian

摘要

Polyploidization is a pivotal process in plant evolution, serving as a major driving force for genome diversification and adaptive potential. However, the meiotic behavior of autoallopolyploid remains poorly understood. Here, we analyzed the meiotic behavior of a Brassica autoallohexaploid (AnAnCnCnCoCo) derived from B. napus (AnAnCnCn) and B. oleracea (CoCo), using fluorescence in situ hybridization (FISH). The addition of the Co genome from B. oleracea induced A-C homoeologous pairing in 78.5% of pollen mother cells (PMCs) in the S0 generation. The prevalence of univalents, autotrivalents, and homoeologous pairings at diakinesis I strongly correlated with irregular chromosomal segregation at anaphase I. The frequency of homoeologous pairing was positively correlated with the extent of chromosomal collinearity. The emergence of a relatively stable euploid line in the S4 generation suggests that successive selfing enhances meiotic stability in autoallohexaploids. Subsequently, we constructed a gene co-expression network based on differentially expressed genes (DEGs) between the stabilized S4 line and the S0 generation, which highlighted hub genes potentially governing meiotic stability. Together, these findings provide new insights into meiotic stability in autoallohexaploid and offer valuable knowledge for polyploid breeding and the improvement of genomic stability in Brassica crops.