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Key message <p>The wheat powdery mildew resistance gene <i>PmPBDH</i> was fine mapped to a 788.20-kb genomic interval on chromosome arm 4AL.</p> Abstract <p>The powdery mildew resistance gene <i>PmPBDH</i> was identified in wheat breeding line PBDH1607. To fine map <i>PmPBDH</i>, an F<sub>2:3</sub> population of 5000 individuals derived from the cross PBDH1607 × Huixianhong was used for recombinant screening. Combining PacBio SMRT long-read genome sequencing, <i>PmPBDH</i> is fine mapped to a 788.20&#xa0;kb interval (chr4A:737865053–738653247&#xa0;bp) based on the reference genome of Chinese Spring (IWGSC RefSeq v2.1). Comparative genomic analysis reveals poor collinearity of this region across different <i>Triticum</i> species, indicating complex structural variations of this candidate interval. Within this interval, 16 high confidence genes are annotated according to IWGSC RefSeq v2.1. Expression profiling following <i>Bgt</i> inoculation and sequence variation analysis reveals <i>TraesCS4A03G1174000</i>, encoding the <i>RPP13</i> nucleotide-binding leucine-rich repeat (NLR) protein, as the pivotal candidate gene for <i>PmPBDH</i>. Three closely linked or co-segregated markers are screened for optimizing the efficient transfer of <i>PmPBDH</i> in breeding practices. This study provides valuable genetic resource and molecular tool for improving resistance against powdery mildew in wheat.</p>

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Fine mapping and candidate gene dissection of the wheat powdery mildew resistance gene PmPBDH

  • 期刊论文

  • 发表日期：

    2026年3月18日

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  • Yuli Jin,
  • Jiatong Li,
  • Delin Li,
  • Ningning Yu,
  • Ya Zhao,
  • Bo Wang,
  • Jun Guo,
  • Jiadong Zhang,
  • Guantong Pan,
  • Yaoxue Li,
  • Yintao Dai,
  • Nina Sun,
  • Linzhi Li,
  • Qingguo Xin,
  • Qiuhong Wu,
  • Guohao Han,
  • Pengtao Ma

摘要

Key message

The wheat powdery mildew resistance gene PmPBDH was fine mapped to a 788.20-kb genomic interval on chromosome arm 4AL.

Abstract

The powdery mildew resistance gene PmPBDH was identified in wheat breeding line PBDH1607. To fine map PmPBDH, an F2:3 population of 5000 individuals derived from the cross PBDH1607 × Huixianhong was used for recombinant screening. Combining PacBio SMRT long-read genome sequencing, PmPBDH is fine mapped to a 788.20 kb interval (chr4A:737865053–738653247 bp) based on the reference genome of Chinese Spring (IWGSC RefSeq v2.1). Comparative genomic analysis reveals poor collinearity of this region across different Triticum species, indicating complex structural variations of this candidate interval. Within this interval, 16 high confidence genes are annotated according to IWGSC RefSeq v2.1. Expression profiling following Bgt inoculation and sequence variation analysis reveals TraesCS4A03G1174000, encoding the RPP13 nucleotide-binding leucine-rich repeat (NLR) protein, as the pivotal candidate gene for PmPBDH. Three closely linked or co-segregated markers are screened for optimizing the efficient transfer of PmPBDH in breeding practices. This study provides valuable genetic resource and molecular tool for improving resistance against powdery mildew in wheat.