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<p>Seed oil content (SOC) of oilseed rape (<i>Brassica napus</i> L.) is an important agricultural trait that is controlled by a complex regulatory mechanism. In this study, we performed integrated analyses of Genome-Wide Association Studies (GWAS) and Target Induced Local Lesions in Genomes (TILLING) to explore genetic loci/mutant alleles associated with SOC in rapeseed. GWAS analysis of a diverse panel of 324 accessions identified 142 SOC-associated quantitative trait loci (QTLs). Of them, <i>qSOC.C06.4</i> encompassed the HD-ZIP transcription factor <i>BnaC.GL2.b</i>, which may regulate oil accumulation predicted by haplotypes analysis. An EMS (ethylmethanesulfonate)-TILLING platform that consists of 4,734 M<sub>2</sub> plants was constructed to identify the mutants of <i>BnaC.GL2.b</i> in rapeseed. Five mutant alleles of <i>BnaC.GL2.b</i> were identified using TILLING, of which three missense mutants exhibited higher SOC than the controls (two nonsense mutants). In addition, we measured SOC of 9332 M<sub>2</sub> plants ranging from 22.06% to 51.00%, with the average of 38.19%. Together, we propose to use GWAS combined with TILLING to identify causal genes and mutant alleles for a quantitative trait in the polyploid rapeseed. Furthermore, our new rapeseed germplasm resources may be useful for mutation breeding.</p>

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Joint analysis of GWAS and TILLING reveals the negative role of GLABRA2 in controlling seed oil content in Brassica napus

  • 期刊论文

  • 发表日期：

    2026年1月24日

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  • Zetao Bai,
  • Haibo Sun,
  • Weidong Shi,
  • Cong Zhou,
  • Ming Hu,
  • Meili Xie,
  • Feng Gao,
  • Chaobo Tong,
  • Yueying Liu,
  • Guohua Chai,
  • Shengyi Liu

摘要

Seed oil content (SOC) of oilseed rape (Brassica napus L.) is an important agricultural trait that is controlled by a complex regulatory mechanism. In this study, we performed integrated analyses of Genome-Wide Association Studies (GWAS) and Target Induced Local Lesions in Genomes (TILLING) to explore genetic loci/mutant alleles associated with SOC in rapeseed. GWAS analysis of a diverse panel of 324 accessions identified 142 SOC-associated quantitative trait loci (QTLs). Of them, qSOC.C06.4 encompassed the HD-ZIP transcription factor BnaC.GL2.b, which may regulate oil accumulation predicted by haplotypes analysis. An EMS (ethylmethanesulfonate)-TILLING platform that consists of 4,734 M2 plants was constructed to identify the mutants of BnaC.GL2.b in rapeseed. Five mutant alleles of BnaC.GL2.b were identified using TILLING, of which three missense mutants exhibited higher SOC than the controls (two nonsense mutants). In addition, we measured SOC of 9332 M2 plants ranging from 22.06% to 51.00%, with the average of 38.19%. Together, we propose to use GWAS combined with TILLING to identify causal genes and mutant alleles for a quantitative trait in the polyploid rapeseed. Furthermore, our new rapeseed germplasm resources may be useful for mutation breeding.