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Key message <p>The natural variation of <i>TaDHN4D1</i>, co-targeted by TaNAC4 and TaWRKY19, influences salt stress tolerance in wheat.</p> Abstract <p>Salt stress is a significant abiotic stressor that negatively affects wheat production. In this study, we elucidated the role of wheat <i>TaDHN4D1</i> in response to salt stress. Our results indicated that the overexpression of <i>TaDHN4D1</i> alters sodium (Na<sup>+</sup>) balance under salt stress and significantly enhances the ability of wheat to eliminate reactive oxygen species (ROS), thereby improving its salt tolerance. Furthermore, we discovered that TaNAC4 binds to the promoter of <i>TaDHN4D1</i>, positively regulating its expression. We also examined the expression levels of <i>TaDHN4D1</i> across various wheat germplasms and found that the expression level of <i>TaDHN4D1</i> in the wheat landrace BD2 (<i>TaDHN4D1</i><sup><i>BD2</i></sup>) was significantly higher than in other wheat varieties. Additionally, we identified sequence variations in the promoter of <i>TaDHN4D1</i><sup><i>BD2</i></sup>, including an additional W-box, which is recognized by TaWRKY19. This interaction suggests that TaNAC4 and TaWRKY19 cotarget the <i>TaDHN4D1</i><sup><i>BD2</i></sup> promoter, regulating the expression of <i>TaDHN4D1</i><sup><i>BD2</i></sup>. Finally, we designed specific primers for <i>TaDHN4D1</i><sup><i>BD2</i></sup> and, through molecular marker-assisted selection, developed salt-tolerant wheat materials containing <i>TaDHN4D1</i><sup><i>BD2</i></sup>. In conclusion, this study identifies a novel mechanism involved in the regulation of salt tolerance in wheat, providing valuable insights for the cultivation and breeding of salt-tolerant wheat.</p>

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A novel natural variation in the promoter of TaDHN4D1 confers salt tolerance in wheat

  • 期刊论文

  • 发表日期：

    2025年12月11日

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  • Zhenzhen Jia,
  • Tuo Zeng,
  • Lei Gu,
  • Hongcheng Wang,
  • Bin Zhu,
  • Mingjian Ren,
  • Xuye Du

摘要

Key message

The natural variation of TaDHN4D1, co-targeted by TaNAC4 and TaWRKY19, influences salt stress tolerance in wheat.

Abstract

Salt stress is a significant abiotic stressor that negatively affects wheat production. In this study, we elucidated the role of wheat TaDHN4D1 in response to salt stress. Our results indicated that the overexpression of TaDHN4D1 alters sodium (Na+) balance under salt stress and significantly enhances the ability of wheat to eliminate reactive oxygen species (ROS), thereby improving its salt tolerance. Furthermore, we discovered that TaNAC4 binds to the promoter of TaDHN4D1, positively regulating its expression. We also examined the expression levels of TaDHN4D1 across various wheat germplasms and found that the expression level of TaDHN4D1 in the wheat landrace BD2 (TaDHN4D1BD2) was significantly higher than in other wheat varieties. Additionally, we identified sequence variations in the promoter of TaDHN4D1BD2, including an additional W-box, which is recognized by TaWRKY19. This interaction suggests that TaNAC4 and TaWRKY19 cotarget the TaDHN4D1BD2 promoter, regulating the expression of TaDHN4D1BD2. Finally, we designed specific primers for TaDHN4D1BD2 and, through molecular marker-assisted selection, developed salt-tolerant wheat materials containing TaDHN4D1BD2. In conclusion, this study identifies a novel mechanism involved in the regulation of salt tolerance in wheat, providing valuable insights for the cultivation and breeding of salt-tolerant wheat.