Scholarlink

<p>Wheat is a vital global crop, essential for food security, but its production is threatened by soil-borne diseases like <i>Fusarium graminearum</i>. This pathogen infects wheat at all growth stages and produces harmful mycotoxins, severely compromising both yield and quality. <i>Piriformospora indica</i>, endophytic fungi of plant rhizosphere, can not only effectively promote growth and development, but also improve disease resistance in plants. Here, the mechanism of Ta<i>CPS1</i> gene expression induced by <i>P. indica</i> colonization to improve wheat resistance to <i>F. graminearum</i> has been investigated<i>.</i> Results showed that colonization of <i>P. indica</i> decreased infection of <i>F. graminearum</i> and correspondingly reduced deoxynivalenol (DON) content in wheat roots. Transcriptome sequence analysis demonstrated colonization of <i>P. indica</i> leads to a high expression of the synthase gene family of diterpenoid metabolites. Ta<i>CPS1</i> gene, a crucial gene in the synthesis of diterpenoid metabolites, responded promptly to the colonization of <i>P. indica</i>. Subsequently, 31 transcription factors were screened by yeast one-hybrid library screening and TaZF-HD5 was verified to be interacted with the promoter of Ta<i>CPS1</i>. The findings indicate that plants overexpression Ta<i>CPS1</i> demonstrated heightened responsiveness to <i>P. indica</i> colonization and exhibited enhanced resistance to root rot. The increased phytocassane content in Ta<i>CPS1</i> overexpression lines implies that Ta<i>CPS1</i> plays positive role in phytoalexin biosynthesis. The upregulated expression of Ta<i>CPS1</i> in response to the colonization of <i>P. indica</i>, thereby increasing the content of phytocassane synthesis, is one of the reasons why <i>P. indica</i> mediates wheat disease resistance to <i>F. graminearum.</i></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Copalyl diphosphate synthase gene TaCPS1 mediated resistance to Fusarium graminearum in wheat in response to Piriformospora indica colonization

  • 期刊论文

  • 发表日期：

    2025年11月26日

查看全文
  • Liang Li,
  • Fuyan Qi,
  • Ruiying Hao

摘要

Wheat is a vital global crop, essential for food security, but its production is threatened by soil-borne diseases like Fusarium graminearum. This pathogen infects wheat at all growth stages and produces harmful mycotoxins, severely compromising both yield and quality. Piriformospora indica, endophytic fungi of plant rhizosphere, can not only effectively promote growth and development, but also improve disease resistance in plants. Here, the mechanism of TaCPS1 gene expression induced by P. indica colonization to improve wheat resistance to F. graminearum has been investigated. Results showed that colonization of P. indica decreased infection of F. graminearum and correspondingly reduced deoxynivalenol (DON) content in wheat roots. Transcriptome sequence analysis demonstrated colonization of P. indica leads to a high expression of the synthase gene family of diterpenoid metabolites. TaCPS1 gene, a crucial gene in the synthesis of diterpenoid metabolites, responded promptly to the colonization of P. indica. Subsequently, 31 transcription factors were screened by yeast one-hybrid library screening and TaZF-HD5 was verified to be interacted with the promoter of TaCPS1. The findings indicate that plants overexpression TaCPS1 demonstrated heightened responsiveness to P. indica colonization and exhibited enhanced resistance to root rot. The increased phytocassane content in TaCPS1 overexpression lines implies that TaCPS1 plays positive role in phytoalexin biosynthesis. The upregulated expression of TaCPS1 in response to the colonization of P. indica, thereby increasing the content of phytocassane synthesis, is one of the reasons why P. indica mediates wheat disease resistance to F. graminearum.