Scholarlink

Key message <p><i>QSes-7AL</i> is the first major QTL for sharp eyespot resistance identified on wheat chromosome 7AL. The putative candidate gene <i>TaPrx1L-7A</i> was identified and preliminarily validated through integrated multi-omics data and molecular biology approaches.</p> Abstract <p>Wheat sharp eyespot is a soilborne disease caused by the necrotrophic fungal pathogen <i>Rhizoctonia cerealis</i> and is a major threat to wheat yield and quality worldwide. In this study, a novel major quantitative trait locus (QTL) for sharp eyespot resistance, designated <i>QSes-7AL</i>, was identified on chromosome 7AL (496.041–499.622 Mb) though analysis of a recombinant inbred line (RIL) population derived from a cross between H63-4 and Yangmai 158, using bulked segregant analysis (BSA), the wheat 660K SNP array, simple sequence repeat (SSR) markers, and kompetitive allele-specific PCR (KASP) markers. A peroxidase-encoding gene, <i>TaPrx1L-7A</i>, was identified as the most likely candidate gene for <i>QSes-7AL</i> based on integrated multi-omics data, gene function annotation, and expression pattern analysis. In addition, the gene was further validated as a strong candidate for <i>QSes-7AL</i> through diagnostic molecular marker, barley stripe mosaic virus-induced gene silencing (BSMV-VIGS), analysis of defense-related gene expression, and measurement of physiological indicators. Overall, these findings provide new insights into the molecular mechanisms underlying wheat resistance to sharp eyespot and offer a theoretical foundation for breeding resistant wheat varieties.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification of genetic loci and candidate genes underlying sharp eyespot resistance in wheat

  • 期刊论文

  • 发表日期：

    2025年11月28日

查看全文
  • Zhen Gao,
  • Linrun Xiang,
  • Miao Sun,
  • Shaowei Jiang,
  • Chunyu Shao,
  • Jie Cai,
  • Xingxia Geng,
  • Xinhong Chen,
  • Jun Wang

摘要

Key message

QSes-7AL is the first major QTL for sharp eyespot resistance identified on wheat chromosome 7AL. The putative candidate gene TaPrx1L-7A was identified and preliminarily validated through integrated multi-omics data and molecular biology approaches.

Abstract

Wheat sharp eyespot is a soilborne disease caused by the necrotrophic fungal pathogen Rhizoctonia cerealis and is a major threat to wheat yield and quality worldwide. In this study, a novel major quantitative trait locus (QTL) for sharp eyespot resistance, designated QSes-7AL, was identified on chromosome 7AL (496.041–499.622 Mb) though analysis of a recombinant inbred line (RIL) population derived from a cross between H63-4 and Yangmai 158, using bulked segregant analysis (BSA), the wheat 660K SNP array, simple sequence repeat (SSR) markers, and kompetitive allele-specific PCR (KASP) markers. A peroxidase-encoding gene, TaPrx1L-7A, was identified as the most likely candidate gene for QSes-7AL based on integrated multi-omics data, gene function annotation, and expression pattern analysis. In addition, the gene was further validated as a strong candidate for QSes-7AL through diagnostic molecular marker, barley stripe mosaic virus-induced gene silencing (BSMV-VIGS), analysis of defense-related gene expression, and measurement of physiological indicators. Overall, these findings provide new insights into the molecular mechanisms underlying wheat resistance to sharp eyespot and offer a theoretical foundation for breeding resistant wheat varieties.