<p>Spikelet number per spike (SNS), a key determinant of grain yield in wheat, is a complex quantitative trait controlled by spike meristem activity. Unraveling its genetic basis is essential for wheat high-yield breeding. Here, we identified a stable quantitative trait locus (QTL), <i>QSNS.sicau-D12-3B</i>, governing SNS using a recombinant inbred line (RIL) population derived from a cross between the high-SNS line 03D501 and the low-SNS line 2011. This QTL was consistently detected across seven environments and mapped to a 5.814&#xa0;Mb interval on chromosome 3B (flanked by markers <i>K_sau_3B_619673521</i> and <i>K_sau_3B_625487833</i>). Conditional QTL analysis revealed that <i>QSNS.sicau-D12-3B</i> acts independently of plant height and spike length but may modulate SNS through its effect on spike density. Validation in diverse genetic backgrounds confirmed the robust association between <i>QSNS.sicau-D12-3B</i> and SNS, underscoring its potential for yield improvement. Our findings provide novel insights into the genetic architecture of SNS and offer a promising target for molecular breeding in wheat.</p>

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QSNS.sicau-D12-3B: A novel and major validated QTL governing wheat spikelet number with significant breeding potential

  • Tongzhu Wang,
  • Xinrong Hu,
  • Lei Yan,
  • Li Yin,
  • Jiating Chen,
  • Guangdeng Chen,
  • Qiang Xu,
  • Qiantao Jiang,
  • Guoyue Chen,
  • Yuming Wei,
  • Chunji Liu,
  • Youliang Zheng,
  • Jian Ma

摘要

Spikelet number per spike (SNS), a key determinant of grain yield in wheat, is a complex quantitative trait controlled by spike meristem activity. Unraveling its genetic basis is essential for wheat high-yield breeding. Here, we identified a stable quantitative trait locus (QTL), QSNS.sicau-D12-3B, governing SNS using a recombinant inbred line (RIL) population derived from a cross between the high-SNS line 03D501 and the low-SNS line 2011. This QTL was consistently detected across seven environments and mapped to a 5.814 Mb interval on chromosome 3B (flanked by markers K_sau_3B_619673521 and K_sau_3B_625487833). Conditional QTL analysis revealed that QSNS.sicau-D12-3B acts independently of plant height and spike length but may modulate SNS through its effect on spike density. Validation in diverse genetic backgrounds confirmed the robust association between QSNS.sicau-D12-3B and SNS, underscoring its potential for yield improvement. Our findings provide novel insights into the genetic architecture of SNS and offer a promising target for molecular breeding in wheat.