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<p>Wheat production is challenged by biotic and abiotic stresses. Alien gene transfer is an effective approach to tackle such challenges. We previously showed that sea wheatgrass (SWG; <i>Thinopyrum junceiforme</i> (2<i>n</i> = 2<i>x</i> = 28; J<sub>1</sub>J<sub>2</sub>) is an untapped resource possessing resistance to an array of pests and abiotic stress. However, the transfer of these important traits has been hindered by the lack of genomic resources and a clear picture of its genome constitution. Using multi-color genomic in situ hybridization, we distinguished the SWG sub-genomes and corroborated that the J<sub>1</sub> sub-genome is closely related to the E genome of <i>Th. elongatum</i> and the J genome of <i>Th. bessarabicum</i> and the J<sub>2</sub> sub-genome to the V genome of <i>Dasypyrum villosum</i>. Meanwhile, we developed a draft SWG genome assembly and 127&#xa0;SWG-specific DNA markers covering the 14&#xa0;SWG chromosomes. Screening a population of 466 BC<sub>2</sub>F<sub>1</sub> and BC<sub>2</sub>F<sub>2</sub> individuals, derived from backcrosses of wheat-SWG amphiploid to wheat, by the SWG-specific markers led to selection of 72 plants putatively carrying one or two SWG chromosomes. The genome painting analysis of the 72 plants eventually identified a set of 37&#xa0;wheat-SWG chromosome addition lines covering all the 14 pairs of SWG chromosomes and two compensating Robertsonian translocations (RobTs). While the wheat-SWG chromosome addition lines and RobTs are invaluable genetic resources for wheat improvement via chromosome engineering, our results showed the power of genome-specific markers in combination with genome painting in dissection of a polyploid genome and implicated the origin of a group of important polyploid grasses.</p>

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Genomics-enabled dissection of sea wheatgrass genome for advancing wheat genetic resources

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月18日

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  • Dilkaran Singh,
  • Qijun Zhang,
  • Ghana Challa,
  • Elias M. Elias,
  • Steven S. Xu,
  • Wanlong Li

摘要

Wheat production is challenged by biotic and abiotic stresses. Alien gene transfer is an effective approach to tackle such challenges. We previously showed that sea wheatgrass (SWG; Thinopyrum junceiforme (2n = 2x = 28; J1J2) is an untapped resource possessing resistance to an array of pests and abiotic stress. However, the transfer of these important traits has been hindered by the lack of genomic resources and a clear picture of its genome constitution. Using multi-color genomic in situ hybridization, we distinguished the SWG sub-genomes and corroborated that the J1 sub-genome is closely related to the E genome of Th. elongatum and the J genome of Th. bessarabicum and the J2 sub-genome to the V genome of Dasypyrum villosum. Meanwhile, we developed a draft SWG genome assembly and 127 SWG-specific DNA markers covering the 14 SWG chromosomes. Screening a population of 466 BC2F1 and BC2F2 individuals, derived from backcrosses of wheat-SWG amphiploid to wheat, by the SWG-specific markers led to selection of 72 plants putatively carrying one or two SWG chromosomes. The genome painting analysis of the 72 plants eventually identified a set of 37 wheat-SWG chromosome addition lines covering all the 14 pairs of SWG chromosomes and two compensating Robertsonian translocations (RobTs). While the wheat-SWG chromosome addition lines and RobTs are invaluable genetic resources for wheat improvement via chromosome engineering, our results showed the power of genome-specific markers in combination with genome painting in dissection of a polyploid genome and implicated the origin of a group of important polyploid grasses.