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Key message <p>Three environmentally stable major QTL controlling seed oil content in peanut were identified, and their genetic effects were evaluated in near‑isogenic lines and a peanut germplasm panel.</p> Abstract <p>Increasing seed oil content (SOC) is a primary objective in peanut (<i>Arachis hypogaea</i> L.) breeding, meeting the rising global demand for edible oil. Quantitative trait loci (QTL) mapping can help to identify genes underlying SOC variation and develop markers to enhance selection efficiency by marker-assisted breeding. In the present study, three major and stable QTL for SOC were identified on peanut chromosome Arahy.08, using a bulked segregant analysis (BSA) approach based on whole-genome sequencing of F<sub>8</sub> recombinant inbred lines (RILs). The QTL <i>qSOCA08-1</i>, explaining 11.41–20.97% of phenotypic variation, was mapped on a 0.65-Mb genomic region. The QTL <i>qSOCA08-2</i>, accounting 25.57–39.40% of phenotypic variation, was located on a 1.04-Mb physical interval. Finally, <i>qSOCA08-3</i> explaining up to 17.31% of the phenotypic variation was mapped in a genomic region of 1.02-Mb. The genetic effects of these three QTL were assessed using near‑isogenic lines (NILs), derived from residual heterozygous individuals, and a tetraploid peanut germplasm panel. Potential candidate genes within the physical intervals of corresponding major QTL were predicted to participate in the oil biosynthesis in peanut. In summary, our study provides valuable genetic resources and tightly linked molecular markers for peanut molecular breeding aimed at improving SOC.</p>

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Integration of BSA-seq and high-resolution mapping reveals genomic regions and candidate genes controlling seed oil accumulation in peanut (Arachis hypogaea L.)

  • 期刊论文

  • 发表日期：

    2025年6月17日

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  • Ziqiang Mo,
  • Feiyan Qi,
  • Ziqi Sun,
  • Li Qin,
  • Juan Wang,
  • Mengmeng Wang,
  • Stefano Pavan,
  • Guoquan Chen,
  • Xiao Wang,
  • Hongfei Liu,
  • Yaojun Hu,
  • Yuzhen Zheng,
  • Zheng Zheng,
  • Xinyou Zhang

摘要

Key message

Three environmentally stable major QTL controlling seed oil content in peanut were identified, and their genetic effects were evaluated in near‑isogenic lines and a peanut germplasm panel.

Abstract

Increasing seed oil content (SOC) is a primary objective in peanut (Arachis hypogaea L.) breeding, meeting the rising global demand for edible oil. Quantitative trait loci (QTL) mapping can help to identify genes underlying SOC variation and develop markers to enhance selection efficiency by marker-assisted breeding. In the present study, three major and stable QTL for SOC were identified on peanut chromosome Arahy.08, using a bulked segregant analysis (BSA) approach based on whole-genome sequencing of F8 recombinant inbred lines (RILs). The QTL qSOCA08-1, explaining 11.41–20.97% of phenotypic variation, was mapped on a 0.65-Mb genomic region. The QTL qSOCA08-2, accounting 25.57–39.40% of phenotypic variation, was located on a 1.04-Mb physical interval. Finally, qSOCA08-3 explaining up to 17.31% of the phenotypic variation was mapped in a genomic region of 1.02-Mb. The genetic effects of these three QTL were assessed using near‑isogenic lines (NILs), derived from residual heterozygous individuals, and a tetraploid peanut germplasm panel. Potential candidate genes within the physical intervals of corresponding major QTL were predicted to participate in the oil biosynthesis in peanut. In summary, our study provides valuable genetic resources and tightly linked molecular markers for peanut molecular breeding aimed at improving SOC.