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Key message <p>A novel Fusarium head blight resistance locus, <i>QFhb-3BL</i>, was identified and fine-mapped in common wheat.</p> Abstract <p>Identification and utilization of major resistance genes is key to developing new wheat cultivars/lines with improved Fusarium head blight (FHB) resistance. In this study, a moderately FHB-resistant cultivar Yangmai 16.2 (YM16.2) was crossed with a susceptible cultivar Yanzhan 1 (YZ1) to create a recombinant inbred line (RIL) population. Using bulk segregant analysis sequencing (BSA-seq) and linkage analysis, a new FHB quantitative trait locus (QTL) named <i>QFhb-3BL</i>, contributed by YM16.2, was identified. Eight Kompetitive Allele-Specific PCR (KASP) markers were used to detect homozygous recombinants in the derived lines of the remaining heterozygous line (RHL). <i>QFhb-3BL</i> was finely mapped to the 751.10–756.45&#xa0;Mb interval, which contains 75 high-confidence genes. Through variation analysis of the candidate region and RNA sequencing (RNA-seq), we identified 11 genes with sequence variations and 14 genes expressed within the fine-mapping interval, with four genes overlapping between the two groups. KASP markers for the candidate gene <i>TraesCS3B03G1226500</i> were validated in 103 wheat cultivars/lines, and lines carrying these markers showed a 38.89% reduction in FHB severity. This study provides a foundation for <i>QFhb-3BL</i> cloning and an effective marker for FHB resistance breeding.</p>

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Identification of a novel QTL associated with Fusarium head blight resistance on chromosome 3B in common wheat

  • 期刊论文

  • 发表日期：

    2025年6月12日

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  • Wenjing Hu,
  • Xiao Zhang,
  • Dongmei Zhu,
  • Die Zhao,
  • Rui Yong,
  • Junchao You,
  • Yong Zhang,
  • Derong Gao

摘要

Key message

A novel Fusarium head blight resistance locus, QFhb-3BL, was identified and fine-mapped in common wheat.

Abstract

Identification and utilization of major resistance genes is key to developing new wheat cultivars/lines with improved Fusarium head blight (FHB) resistance. In this study, a moderately FHB-resistant cultivar Yangmai 16.2 (YM16.2) was crossed with a susceptible cultivar Yanzhan 1 (YZ1) to create a recombinant inbred line (RIL) population. Using bulk segregant analysis sequencing (BSA-seq) and linkage analysis, a new FHB quantitative trait locus (QTL) named QFhb-3BL, contributed by YM16.2, was identified. Eight Kompetitive Allele-Specific PCR (KASP) markers were used to detect homozygous recombinants in the derived lines of the remaining heterozygous line (RHL). QFhb-3BL was finely mapped to the 751.10–756.45 Mb interval, which contains 75 high-confidence genes. Through variation analysis of the candidate region and RNA sequencing (RNA-seq), we identified 11 genes with sequence variations and 14 genes expressed within the fine-mapping interval, with four genes overlapping between the two groups. KASP markers for the candidate gene TraesCS3B03G1226500 were validated in 103 wheat cultivars/lines, and lines carrying these markers showed a 38.89% reduction in FHB severity. This study provides a foundation for QFhb-3BL cloning and an effective marker for FHB resistance breeding.