Scholarlink

Key message <p>The tomato <i>Ty-6</i> gene conferring resistance against begomoviruses has been cloned and shown to be a variant of DNA polymerase delta subunit 1.</p> Abstract <p><i>Ty-6</i> is a major resistance gene of tomato that provides resistance against monopartite and bipartite begomoviruses. The locus was previously mapped on chromosome 10, and in this study, we fine-mapped <i>Ty-6</i> to a region of 47&#xa0;kb, including four annotated candidate genes. Via whole-genome resequencing of <i>Ty-6</i> breeding lines and several susceptible breeding lines, the polymorphisms in gene sequences were discovered and gene-associated markers were developed for marker-assistant breeding. Further, virus-induced gene silencing and candidate gene overexpressing in susceptible tomatoes revealed that <i>Ty-6-</i>mediated resistance is controlled by Solyc10g081250, encoding the DNA polymerase delta subunit 1, <i>SlPOLD1</i>. The single nucleotide polymorphism of <i>Ty-6</i> results in an amino acid change that might influence the fidelity of virus DNA replication.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The tomato gene Ty-6, encoding DNA polymerase delta subunit 1, confers broad resistance to Geminiviruses

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月8日

查看全文
  • Xuexue Shen,
  • Upinder Gill,
  • Marjon Arens,
  • Zhe Yan,
  • Yuling Bai,
  • Samuel F. Hutton,
  • Anne-Marie A. Wolters

摘要

Key message

The tomato Ty-6 gene conferring resistance against begomoviruses has been cloned and shown to be a variant of DNA polymerase delta subunit 1.

Abstract

Ty-6 is a major resistance gene of tomato that provides resistance against monopartite and bipartite begomoviruses. The locus was previously mapped on chromosome 10, and in this study, we fine-mapped Ty-6 to a region of 47 kb, including four annotated candidate genes. Via whole-genome resequencing of Ty-6 breeding lines and several susceptible breeding lines, the polymorphisms in gene sequences were discovered and gene-associated markers were developed for marker-assistant breeding. Further, virus-induced gene silencing and candidate gene overexpressing in susceptible tomatoes revealed that Ty-6-mediated resistance is controlled by Solyc10g081250, encoding the DNA polymerase delta subunit 1, SlPOLD1. The single nucleotide polymorphism of Ty-6 results in an amino acid change that might influence the fidelity of virus DNA replication.