<p>Fresh leaves of <i>Wrightia tinctoria</i> plant is used by traditional healers in India for wound healing. Even though few research studies conducted on the wound healing property of dry leaves, no scientific proof available for fresh leaf extract of <i>W. tinctoria</i>. The present work aims to analyse the phytochemical components in the fresh leaves of <i>W. tinctoria</i> and also to investigate the wound healing potential in excision wound model. The methanolic extract of fresh leaves possess high levels of phytochemical compounds and the GC-MS analysis indicated the presence of major phytochemicals 8-prenylnaringenin, bilobalide and trans-cinnamic acid. Methanolic extract recorded superior antioxidant, anti-inflammatory and antibacterial activities than other solvent extracts and also protected from intracellular generation of ROS in PBMC cells. Further, it induced angiogenesis in CAM model and found to be safe based on MTT assay. In vivo studies revealed that ointment prepared from methanolic extract of <i>W. tinctoria</i> fresh leaf healed the wound faster (100%) than betadine-treated on 13th day. Hence, methanolic extract of fresh leaves of <i>W. tinctoria</i> could be explored as potential candidate for the development of green and safe wound healing drug.</p>