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Purpose <p>Interleukin (IL)-17 expression in the periodontal ligament is associated with orthodontically induced inflammatory root resorption (OIIRR). Seeking a&#xa0;convenient, rapid, and non-invasive IL-17 detection approach could help predict OIIRR. In this study, we assessed the potential of the IL-17 level in gingival crevicular fluid (GCF) to be an indicator of OIIRR.</p> Methods <p>To enable tooth movement, 8‑ to 10-week-old male Wistar rats were subjected to 20 g/60 g orthodontic force for up to 14&#xa0;days. GCF and peripheral blood were collected for the IL-17 enzyme-linked immunosorbent assay (ELISA). Histological changes and the expression of IL-17, receptor activator of nuclear factor&#xa0;κB ligand (RANKL), and osteoprotegerin (OPG) in periodontal tissue were evaluated by hematoxylin–eosin and immunohistochemistry staining, respectively. Osteoclasts were visualized by tartrate-resistant acid phosphatase (TARP) staining.</p> Results <p>GCF IL-17 level rapidly rose in the early phase of orthodontic loading, accompanied by bone and tooth root destruction. At the later stage, the GCF IL-17 level gradually decreased, while the inflammatory destruction was reduced and the periodontal tissue began to repair. GCF IL-17 expression generally tended to be coincident with the periodontal tissue reaction: GCF IL-17 was robustly correlated to the RANKL/OPG ratio (r<sub>s</sub> = 0.72, <i>p</i> = 0.002) or osteoclast number (r<sub>s</sub> = 0.84, <i>p</i> &lt; 0.001). Additionally, the GCF IL-17 level was correlated to serum IL-17 (r<sub>s</sub> = 0.61, <i>p</i> = 0.016) or periodontal IL-17 (r<sub>s</sub> = 0.84, <i>p</i> &lt; 0.001).</p> Conclusion <p>The IL-17 level in GCF during orthodontic treatment is associated with OIIRR and, thus, could be an early indicator for developing OIIRR. The advantages of being a&#xa0;convenient, rapid, and non-invasive GCF IL-17 measurement make it a&#xa0;promising method for early detection of OIIRR.</p>

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The IL-17 level in gingival crevicular fluid as an indicator of orthodontically induced inflammatory root resorption

  • 期刊论文

  • 发表日期：

    2025年1月21日

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  • Honghong Zhang,
  • Lufei Wang,
  • Aipeng Liu,
  • Hua Zhou,
  • Xiao Liang,
  • Na Kang

摘要

Purpose

Interleukin (IL)-17 expression in the periodontal ligament is associated with orthodontically induced inflammatory root resorption (OIIRR). Seeking a convenient, rapid, and non-invasive IL-17 detection approach could help predict OIIRR. In this study, we assessed the potential of the IL-17 level in gingival crevicular fluid (GCF) to be an indicator of OIIRR.

Methods

To enable tooth movement, 8‑ to 10-week-old male Wistar rats were subjected to 20 g/60 g orthodontic force for up to 14 days. GCF and peripheral blood were collected for the IL-17 enzyme-linked immunosorbent assay (ELISA). Histological changes and the expression of IL-17, receptor activator of nuclear factor κB ligand (RANKL), and osteoprotegerin (OPG) in periodontal tissue were evaluated by hematoxylin–eosin and immunohistochemistry staining, respectively. Osteoclasts were visualized by tartrate-resistant acid phosphatase (TARP) staining.

Results

GCF IL-17 level rapidly rose in the early phase of orthodontic loading, accompanied by bone and tooth root destruction. At the later stage, the GCF IL-17 level gradually decreased, while the inflammatory destruction was reduced and the periodontal tissue began to repair. GCF IL-17 expression generally tended to be coincident with the periodontal tissue reaction: GCF IL-17 was robustly correlated to the RANKL/OPG ratio (rs = 0.72, p = 0.002) or osteoclast number (rs = 0.84, p < 0.001). Additionally, the GCF IL-17 level was correlated to serum IL-17 (rs = 0.61, p = 0.016) or periodontal IL-17 (rs = 0.84, p < 0.001).

Conclusion

The IL-17 level in GCF during orthodontic treatment is associated with OIIRR and, thus, could be an early indicator for developing OIIRR. The advantages of being a convenient, rapid, and non-invasive GCF IL-17 measurement make it a promising method for early detection of OIIRR.