Anatomy, histochemistry, and phytochemistry of Apeiba tibourbou (Aubl.) leaves and their possible influence on plant-insect interactions
摘要
The present study aimed to evaluate the anatomical, histochemical, and phytochemical characteristics of Apeiba tibourbou (Aubl.) leaves, analyzing their possible relationship with herbivory. The anatomical characterization was carried out using histological slides prepared with freehand transverse sections in the middle region of the leaf blade, stained with fuchsin and Astra blue. The presence of secondary metabolites was determined through histochemical analyses using histological sections of the plant material, which were subjected to preestablished histochemical tests. Phytochemical analyses were conducted using alcoholic extracts from the dried and ground plant material, which were subjected to specific tests for group of metabolite of interest. The anatomical description revealed the presence of glandular trichomes and calcium oxalates that could serve a protective function against herbivory. Histochemical and phytochemical analyses of A. tibourbou leaves revealed the presence of phenolic compounds, alkaloids, saponins and sesquiterpene lactones. Based on the results, A. tibourbou may exhibit potential insecticidal activity against herbivorous insects.