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<p>Bacterial biofilms are surface-attached communities comprised of slow- or non-replicating bacteria. Transcriptomic responses of bacterial biofilms to anti-biofilm small molecules have been largely unexplored, with existing studies typically involving long treatment periods (&gt;18 h). In this study, we used a halogenated quinoline biofilm-killing agent (RA-HQ-12) to investigate the transcriptional responses of MRSA and <i>S. epidermidis</i> biofilms. Utilizing RT-qPCR, we observed RA-HQ-12 activated iron uptake pathways in both MRSA and <i>S. epidermidis</i> biofilms after 4 h treatment at 1 µM. A time-course analysis further revealed dynamic variation in up- and down-regulation patterns of various target genes (<i>sbnC</i>, <i>isdB</i>, <i>opp1C</i>, <i>ribA</i>, <i>nasE</i>, and <i>crtM</i>), shedding light on the time-dependent dynamics of biofilm responses to RA-HQ-12.</p><p></p>

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Halogenated quinoline kill agent rapidly induces iron starvation of Staphylococcal biofilms

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月16日

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  • Robert W. Huigens III,
  • Ke Liu,
  • Nana Shao,
  • Qiwen Gao

摘要

Bacterial biofilms are surface-attached communities comprised of slow- or non-replicating bacteria. Transcriptomic responses of bacterial biofilms to anti-biofilm small molecules have been largely unexplored, with existing studies typically involving long treatment periods (>18 h). In this study, we used a halogenated quinoline biofilm-killing agent (RA-HQ-12) to investigate the transcriptional responses of MRSA and S. epidermidis biofilms. Utilizing RT-qPCR, we observed RA-HQ-12 activated iron uptake pathways in both MRSA and S. epidermidis biofilms after 4 h treatment at 1 µM. A time-course analysis further revealed dynamic variation in up- and down-regulation patterns of various target genes (sbnC, isdB, opp1C, ribA, nasE, and crtM), shedding light on the time-dependent dynamics of biofilm responses to RA-HQ-12.