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<p>Chicken primordial germ cells (PGCs) are valuable for breeding, germplasm preservation, and genetic modifications. Here, using single-cell transcriptome analysis, we found that three transcriptionally distinct PGC subtypes were exist in chicken embryonic gonads PGCs. We further demonstrated that <i>in vitro</i>-cultured PGCs could integrate into recipient gonads via blood vessel injection. Additionally, the gene-modified PGCs could be re-established and integrated into recipient gonads after the PGCs were cultured for long-term use by a second blood-vessel injection. Together, these results provide evidence for substantial heterogeneity among chicken gonadal PGCs, which were previously considered a homogeneous cell population. This diversity could be leveraged to establish and expand PGCs for long-term <i>in vitro</i> culture from differentiated gonads.</p>

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Deciphering the heterogeneity of chicken gonadal primordial germ cells via single-cell RNA sequencing

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月22日

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  • Xiaomin Wang,
  • Shenghu Zhang,
  • Kaipeng Wang,
  • Qianru Li,
  • Yihang Yang,
  • Xingnan Huang,
  • Zhuangfei Wang,
  • Mengmeng Guo,
  • Bo Chen,
  • Zheting Zhang,
  • Bo Wang,
  • Duanqing Pei,
  • Chengchen Zhao

摘要

Chicken primordial germ cells (PGCs) are valuable for breeding, germplasm preservation, and genetic modifications. Here, using single-cell transcriptome analysis, we found that three transcriptionally distinct PGC subtypes were exist in chicken embryonic gonads PGCs. We further demonstrated that in vitro-cultured PGCs could integrate into recipient gonads via blood vessel injection. Additionally, the gene-modified PGCs could be re-established and integrated into recipient gonads after the PGCs were cultured for long-term use by a second blood-vessel injection. Together, these results provide evidence for substantial heterogeneity among chicken gonadal PGCs, which were previously considered a homogeneous cell population. This diversity could be leveraged to establish and expand PGCs for long-term in vitro culture from differentiated gonads.