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Background <p><i>Alveolar echinococcosis</i> (AE) is a fatal zoonotic parasitic disease with biological characteristics similar to cancer. Although previous studies have reported immune dysfunction of Natural Killer (NK) cells due to other inhibitory receptors in AE, limited research has been conducted on the role of Natural Killer cell protein Group 2-A (NKG2A) in human NK cells.</p> Methods and results <p>Our study revealed upregulation of NKG2A expression in peripheral blood and liver tissue NK cells in patients with AE, which was accompanied by a decrease in the secretion of IFN-γ, TNF-α, and Granzyme B by these NK cells. When we blocked the NKG2A receptor during co-culture of NK cells with <i>Echinococcus multilocularis</i> (<i>E. multilocularis</i>) proteins in vitro, we observed increased secretion of IFN-γ, TNF-α, and Granzyme B by NK cells. This observation was further confirmed in an <i>E. multilocularis</i>-infected mice model, in which higher expression levels of NKG2A on NK cells were detected, accompanied by a reduction in IFN-γ, TNF-α and Granzyme B secretion from NK cells. Deletion of NK cells in an <i>E. multilocularis</i>-infected mice model clearly resulted in more aggressive disease progression. Conversely, blocking NKG2A on NK cells results in increased secretion of TNF-α, and Granzyme B by NK cells. Similar changes in NKG2A expression and NK cell quality were observed in a mice model of splenic NK cells, as well as the functional recovery of NK cells after blocking NKG2A.</p> Conclusion <p>Our results demonstrate the involvement of NKG2A in impaired NK cell function during <i>E. multilocularis</i> infection in both humans and mice, suggesting that targeting NKG2A through blockade has the potential to restore NK cell function against this infection.</p>

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Blocking NKG2A in Echinococcus multilocularis infection partially relieves impairment of NK cell function of the host

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月2日

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  • Ayinuer Aierken,
  • Aili Aierken,
  • Kalibixiati Aimulajiang,
  • Abuduaini Abulizi,
  • Zhongdian Yuan,
  • Chang Liu,
  • Dalong Zhu,
  • Hanyue Zhao,
  • Tuerganaili Aji

摘要

Background

Alveolar echinococcosis (AE) is a fatal zoonotic parasitic disease with biological characteristics similar to cancer. Although previous studies have reported immune dysfunction of Natural Killer (NK) cells due to other inhibitory receptors in AE, limited research has been conducted on the role of Natural Killer cell protein Group 2-A (NKG2A) in human NK cells.

Methods and results

Our study revealed upregulation of NKG2A expression in peripheral blood and liver tissue NK cells in patients with AE, which was accompanied by a decrease in the secretion of IFN-γ, TNF-α, and Granzyme B by these NK cells. When we blocked the NKG2A receptor during co-culture of NK cells with Echinococcus multilocularis (E. multilocularis) proteins in vitro, we observed increased secretion of IFN-γ, TNF-α, and Granzyme B by NK cells. This observation was further confirmed in an E. multilocularis-infected mice model, in which higher expression levels of NKG2A on NK cells were detected, accompanied by a reduction in IFN-γ, TNF-α and Granzyme B secretion from NK cells. Deletion of NK cells in an E. multilocularis-infected mice model clearly resulted in more aggressive disease progression. Conversely, blocking NKG2A on NK cells results in increased secretion of TNF-α, and Granzyme B by NK cells. Similar changes in NKG2A expression and NK cell quality were observed in a mice model of splenic NK cells, as well as the functional recovery of NK cells after blocking NKG2A.

Conclusion

Our results demonstrate the involvement of NKG2A in impaired NK cell function during E. multilocularis infection in both humans and mice, suggesting that targeting NKG2A through blockade has the potential to restore NK cell function against this infection.