Scholarlink

Background <p>Sjögren disease (SjD) has a multifactorial pathogenesis that is not fully understood. Perceptions of disease severity shape healthcare-seeking behavior and engagement with diagnostic assessments, underscoring the need for objective biomarkers. Sialic acid-binding immunoglobulin-like lectins (siglecs) have emerged as relevant mediators in SjD immunopathology.</p> Purpose <p>To investigate the diagnostic performance of siglec-5 expression in minor salivary gland (MSG) tissue and saliva samples from individuals with SjD and non-Sjögren <i>sicca</i> (nSS).</p> Methods <p>A total of 109 participants with SjD and 41 with nSS were included. Salivary concentrations of siglec-5/siglec-14, inflammatory markers (IL-6, IL-8, IFN-γ, IgA, IgG, nitric oxide [NO]), and neutrophil extracellular traps (NETs) were measured. Immunohistochemical analyses of siglec-5, CD20, and CD3 were performed on MSG specimens. The data were analyzed descriptively and analytically.</p> Results <p>Salivary levels of siglec-5/siglec-14, IgA, IgG, NO, and NETs were significantly higher in the SjD group compared to the nSS group. Elevated salivary levels of siglec-5/siglec-14, IL-6, and IgG were found among individuals with severe dryness scores. Immunohistochemical staining for siglec-5 was more pronounced in SjD samples and significantly associated with CD20 and CD3 positivity as well as the presence of xerophthalmia. Tissue infiltration by siglec-5 had greater diagnostic accuracy for SjD (area under the curve: 73.1% [95% confidence interval: 58.2–85]) than both salivary and ocular <i>sicca</i> tests.</p> Conclusion <p>Siglec-5 expression was increased in individuals with SjD, supporting its involvement in disease pathogenesis as well as its potential usefulness as a biomarker. The availability of objective salivary and tissue markers may improve diagnostic pathways for SjD, thereby facilitating patient engagement.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Diagnostic significance of salivary and glandular siglec-5 in Sjögren disease and non-Sjögren sicca

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月26日

查看全文
  • Fernanda Luiza Araújo de Lima Castro,
  • Laiz Fernandes Mendes Nunes,
  • Fernanda Aragão Felix,
  • Sicília Rezende Oliveira,
  • José Alcides Almeida de Arruda,
  • Victor Zanetti Drumond,
  • Lucas Guimarães Abreu,
  • Anna Christina Higino Rocha,
  • Camila Munayer Lara,
  • Harim Tavares Dos Santos,
  • Maurício Augusto Aquino de Castro,
  • Gilda Aparecida Ferreira,
  • Leandro Augusto Tanure,
  • Débora Cerqueira Calderaro,
  • Tarcília Aparecida Silva,
  • Sílvia Ferreira de Sousa

摘要

Background

Sjögren disease (SjD) has a multifactorial pathogenesis that is not fully understood. Perceptions of disease severity shape healthcare-seeking behavior and engagement with diagnostic assessments, underscoring the need for objective biomarkers. Sialic acid-binding immunoglobulin-like lectins (siglecs) have emerged as relevant mediators in SjD immunopathology.

Purpose

To investigate the diagnostic performance of siglec-5 expression in minor salivary gland (MSG) tissue and saliva samples from individuals with SjD and non-Sjögren sicca (nSS).

Methods

A total of 109 participants with SjD and 41 with nSS were included. Salivary concentrations of siglec-5/siglec-14, inflammatory markers (IL-6, IL-8, IFN-γ, IgA, IgG, nitric oxide [NO]), and neutrophil extracellular traps (NETs) were measured. Immunohistochemical analyses of siglec-5, CD20, and CD3 were performed on MSG specimens. The data were analyzed descriptively and analytically.

Results

Salivary levels of siglec-5/siglec-14, IgA, IgG, NO, and NETs were significantly higher in the SjD group compared to the nSS group. Elevated salivary levels of siglec-5/siglec-14, IL-6, and IgG were found among individuals with severe dryness scores. Immunohistochemical staining for siglec-5 was more pronounced in SjD samples and significantly associated with CD20 and CD3 positivity as well as the presence of xerophthalmia. Tissue infiltration by siglec-5 had greater diagnostic accuracy for SjD (area under the curve: 73.1% [95% confidence interval: 58.2–85]) than both salivary and ocular sicca tests.

Conclusion

Siglec-5 expression was increased in individuals with SjD, supporting its involvement in disease pathogenesis as well as its potential usefulness as a biomarker. The availability of objective salivary and tissue markers may improve diagnostic pathways for SjD, thereby facilitating patient engagement.