In this paper, we investigate the effects of ντ and \( {\overline{\nu}}_{\tau } \) detection at the DUNE far detector on the experiment’s sensitivity to Non-Standard Interactions (NSI) in neutrino propagation. We show that the strongest observable NSI effect in the ντ and \( {\overline{\nu}}_{\tau } \) appearance probabilities arises from ϵμτ. We have studied the hierarchy sensitivity, CP violation sensitivity and octant sensitivity of DUNE from ντ and \( {\overline{\nu}}_{\tau } \) appearance channels in presence of NSI. We have also studied the detection sensitivity of NSI phases and the future constaints on NSI parameters from the tau neutrino appearance channels in DUNE. Additionally, we examine the role of ντ detection in constraining the unitary nature of the PMNS matrix. These studies emphasize the importance of incorporating ντ detection in long-baseline neutrino experiments such as DUNE.